yeasts, especially saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. the aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. in this ...

We assessed the accuracy of species-level identification of two commercially available matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Bruker Biotyper and Vitek MS) and two conventional phenotypic methods (Phoenix 100 YBC and Vitek 2 Yeast ID) with that of rDNA gene sequencing analysis among 200 clinical isolates of commonly encountered yeast...

MALDI-TOF MS-based yeast identification requires a pretreatment step for which four are described in the literature, i.e., direct smear, fast formic acid and two complete formic acid/acetonitrile extractions. In this study we compared the impact of these procedures on the performance of MALDI-TOF MS-based yeast identification of samples from colonies grown on Sabouraud or chromogenic media. A t...

Candida spp especially non albicans Candida are increasingly being isolated from clinical specimens. The conventional methods of identification are time consuming and difficult to perform. The study was done to evaluate the performance of conventional identification method (phenotypic and biochemical) and commercially available chromogenic Candida speciation media (CHROM agar) for the identific...

lipophilic yeast of the genus malassezia (pityrosporum) belongs to the normal flora of human skin and many warm-blooded animals. these fungi can produce a diverse range of diseases that the best known and most frequent of them is pityriasis versicolor, a chronic recurrent skin disease occurring primarily in tropical regions. the genus malassezia has been recently enlarged to include seven disti...

Yeast is the primary organism responsible for leavening of doughs. In this study, yeast samples were isolated from palm wine collected Nkpa in Abia State. The cultured on malt extract agar (MEA) medium at room temperature 28 o C to isolate three ascosporogenous strains ( Saccharomyces fragilis, cerevisiae, Pichia spp ) culture by using identification techniques such as their morphological, ferm...

BACKGROUND The exacting nutritional requirements and complicated life cycles of parasites mean that they are not always amenable to high-throughput drug screening using automated procedures. Therefore, we have engineered the yeast Saccharomyces cerevisiae to act as a surrogate for expressing anti-parasitic targets from a range of biomedically important pathogens, to facilitate the rapid identif...

background and objectives: acetic acid bacteria (aab) are useful in industrial production of vinegar. the present study aims at isolation and identification of acetic acid bacteria with characterization, optimization, and evaluation of their acetic acid productivity. materials and methods: samples from various fruits were screened for presence of acetic acid bacteria on glucose, yeast extract, ...

Chromogenic Candida Agar is a novel differential culture medium that is claimed to facilitate isolation and identification of Candida albicans, Candida tropicalis and Candida krusei. The performance of this medium was evaluated for presumptive identification of 521 yeast strains, representing 23 different species, for detection of specimens containing yeast mixtures, and for direct isolation of...