Six inbred lines of Zea mays expressing different soluble (cytosolic) malate dehydrogenase (sMDH) zymogram phenotypes were analyzed genetically. sMDH was found to be coded for by unlinked duplicated loci in four of these inbred lines. The remaining two lines were found not to possess these duplicated loci. Furthermore, the duplicated loci, sMdh1 and sMdh2, have been found to be located on diffe...

Eleven enzymes were studied in human malignant mela noma tissue by starch gel electrophoresis. followed by zymogram staining. The results indicate that the frequency of altered enzymes is quite high in this tumor. In a total of 21 human malignant melanoma tumor samples. 6 of 9 different electrophoretically detectable enzymes studied showed alteration in at least 1 or more tumor samples. These p...

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

One hundred and three Aspergillus isolates belonging to 12 species including: A. alliaceus, A. candidus, A. carneus, A. flavus, A. fumigatus, A. niger var. niger, A. niger var. awamori, A. niveus, A. ochraceous, A. sydowii, A. terreus, A. ustus,and A. versicolor based on morphological characters were obtained from various sources. 

The glutamate oxaloacetate transaminase (GOT) zymogram phenotypes of a series of 15 translocation lines, a chromosome addition line and a chromosome substitution line were determined. In each of the translocation lines a segment of the long arm of Triticum aestivum chromosome 3D has been replaced by a portion of an Agropyron elongatum homoeologue. Evidence was obtained that the products of the ...

Xylan-degrading enzymes were induced when Phanerochaete chrysosporium was grown at 30 degrees C in shake flask media containing xylan, Avicel PH 102, or ground corn stalks. The highest xylanase activity was produced in the corn stalk medium, while the xylan-based fermentation resulted in the lowest induction. Analytical and preparative isoelectric focusing were used to characterize xylanase mul...

A genetic approach was cited for species detection of the ameba genus Naegleria using allozyme electrophoresis to characterize the trophozoite stage of three strains of Naegleria fowleri isolated from patients with primary amebic meningoencephalitis, five thermophilic (45 degrees C) Naegleria spp isolated from natural water sources in the Taling Chan district, and a reference control strain, Na...

The appearance of electrophoretically separable isozyme patterns is specific both to type of tissue and to its stage of development (Markert & Moller, 1959). In abnormally developing embryonic systems there are alterations in the time of initial appearance and the persistence of isozymes from a variety of metabolic areas (Johnson & Spinuzzi, 1966, 1968). In malformed embryos from folic-aciddefi...