This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DC...

The green fluorescent protein (GFP) and related genetically-encoded fluorescent proteins have had a major impact in cell biology. GFP has diverse applications in studies of protein localization, dynamics, interactions and regulation. GFP is targetable to specific cellular sites in cell culture models and in vivo in a wide variety of organisms. A unique application of fluorescent proteins is the...

We present ⦇λ⦈, a calculus with special constructions for dealing effects and handlers. This is an extension of the simply-typed λ-calculus (STLC). enrich STLC type representing effectful computations alongside operations to create process values this type. The motivated by natural language modelling, especially semantic representation. Traditionally, meaning sentence calculated using λ-terms, ...

Suppose G is an nvertex and medge simple graph with edge set E(G). An integervalued function f: E(G) → Z is called a flow. Tutte was introduced the flow polynomial F(G, λ) as a polynomial in an indeterminate λ with integer coefficients by F(G,λ) In this paper the Flow polynomial of some dendrimers are computed.