Ku is a heterodimeric protein with high binding affinity for ends, nicks, and gaps in double-stranded DNA. Both in mammalian cells and in budding yeast, Ku plays a role in nonhomologous end joining in the double strand break repair pathway. However, Ku has a more significant role in DNA repair in mammalian cells compared with yeast, in which a homology-dependent pathway is the predominant one. ...

We have investigated whether the Ku complex is involved in regulating DNA replication in the yeast Saccharomyces cerevisiae. We find that Ku proteins control the replication time of telomeric regions; replication origins located close to telomeres or within subtelomeric repeat sequences normally initiate late, but are activated much earlier in mutants lacking Ku function. In contrast, origins d...

1 Department of Neuropsychiatry, School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan 2 Department of Physiology, School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan 3 Department of Child Neuropsychiatry, School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan 4 Department of Radiolog...

In this paper, we introduce a new algebraic structure, called a UP-algebra (UP means the University of Phayao) and a concept of UP-ideals, UP-subalgebras, congruences and UP-homomorphisms in UP-algebras, and investigated some related properties of them. We also describe connections between UP-ideals, UP-subalgebras, congruences and UP-homomorphisms, and show that the notion of UP-algebras is a ...

PURPOSE The purpose of this study was to establish the diagnostic decision point (DDP) of peanut specific IgE (sIgE) for predicting the outcome of oral food challenge (OFC). We also evaluated the usefulness of sIgE to peanut components (Ara h 1, 2, 3, 8, and 9) in diagnosing peanut allergy. METHODS Korean children aged over 12 months with a suspected peanut allergy were enrolled. Diagnosis of...

Nonhomologous end-joining (NHEJ) is an important pathway for the repair of DNA double-strand breaks (DSBs) and plays a critical role in maintaining genomic stability in mammalian cells. While Ku70/80 (Ku) functions in NHEJ as part of the DNA-dependent protein kinase (DNA-PK), genetic evidence indicates that the role of Ku in NHEJ goes beyond its participation in DNA-PK. Inositol hexakisphosphat...

iliZTculty ofAlrpliedBiologicalSciencas, 7bkyo Uhiver:sity ofAgriculture, Setagaya-ku, 7bkyo 156-85(]2, lapan 2Centra1 Reseanch Laboratorieeg Ajinomoto Co,, lhc,, Kawasaki-ku, Kdwasaki-shi, Kbenagawa 210-8681, Jbpan 3FVrstDepartment ofMedieine. Kyoto Ptefectural Uhiversity ofMedicine, Kamigyo-ku, K),oto-city, Kyoto 6(]2-8566; .lapan 4institute for Hlealth Ckere Science, Suntorly Ltd., Shimamoto...

1 Department of Nano-Medical Science, Graduate School of Medicine, Tohoku University, Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan 2 Department of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan 3 Department of Pathology, Tohoku University Hospital, Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan 4 Department...

1 Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan 2 Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan 3 ERATO-SORST Nanospace Project, Japan Science and Technology Agency, 2-41 Aomi, Koto-ku, Tokyo 135-0064, Japan 4 Department of Chemistry and Biotech...

BACKGROUND AND PURPOSE Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks, and trigger a DNA repair pathway. To investigate the involvement of these proteins in DNA fragmentation after ischemia/reperfusion, Ku protein expression was examined before and after transient focal cerebral ischemia (FCI) in mice. METHODS Adult male CD-1 mice w...