Pseudomonas putida KT2440 grows in M9 minimal medium with glucose in the presence of 2,4,6-trinitrotoluene (TNT) at a similar rate than in the absence of TNT, although global transcriptional analysis using DNA microarrays revealed that TNT exerts some stress. Response to TNT stress is regulated at the transcriptional level, as significant changes in the level of expression of 65 genes were obse...

BACKGROUND Cardiac troponin T is independently associated with cardiovascular events and mortality in patients with chronic kidney disease (CKD). Serum levels of high sensitivity cardiac troponin T (hs-TnT) reflect subclinical myocardial injury in ambulatory patients. We sought to determine the distribution and predictors of hs-TnT in CKD patients without overt cardiovascular disease (CVD). M...

Multiple nucleotide sequences of complementary DNA (cDNA) of bovine troponin T (TnT) isoforms expressed in the adult skeletal muscles were determined to facilitate the elucidation of the TnT degradation progress during postmortem aging of muscles. Fresh muscle samples were excised from the lingual, masseter, pectoralis, diaphragm, psoas major, longissimus thoracis, spinnalis, semitendinosus, se...

We have tested the hypothesis whether proteins with biochemical and immunochemical properties similar to those of troponin T (TnT) and troponin I (TnI) are expressed in bovine vascular smooth muscle (SM). Three monoclonal anti-TnT antibodies (TT-1, TT-2, and RV-C2) specific for the two isoforms of TnT present in the bovine cardiac muscle and two monoclonal antibodies (TI-1 and TI-5) reacting wi...

It has previously been demonstrated that 2,4,6-trinitrotoluene (TNT) exhibits characteristic reactivity with negative reactant ions generated in air or O2 during atmospheric-pressure chemical ionization (APCI). For instance, the reaction products were TNT? • (m/z 227) for O2? •, [TNT?H•]? 226) NO3?, and [TNT ? OH•]? 210) NO•]? 197) O3? •. In this work, reactions of TNT atmospheric pressure CO2 ...

OBJECTIVES This study investigated the prognostic value of detectable cardiac troponin T (TnT) and elevated N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels in a population of community-dwelling older adults. BACKGROUND Minimally elevated levels of TnT, a marker of cardiomyocyte injury, have been found in small subsets of the general population, with uncertain implications. A mark...

Post-blast residues are potential interferents to chemical detection of landmines. To assess the potential problem related to 2,4,6-trinitrotoluene (TNT), its post-blast residue was identified and quantified. In the first part of this study laboratory-scale samples of TNT (2 g) were detonated in a small-scale explosivity device (SSED) to evaluate the explosive power and collect post-blast resid...

Purified CO dehydrogenase (CODH) from Clostridium thermoaceticum catalyzed the transformation of 2,4,6-trinitrotoluene (TNT). The intermediates and reduced products of TNT transformation were separated and appear to be identical to the compounds formed by C. acetobutylicum, namely, 2-hydroxylamino-4,6-dinitrotoluene (2HA46DNT), 4-hydroxylamino-2,6-dinitrotoluene (4HA26DNT), 2, 4-dihydroxylamino...

Titania nanotubes (TNT) prepared by anodization of Ti foils were used for water splitting in a standalone cell. The concentration polarization between the anode side (1M NaOH) and cathode side (0.5 M H2SO4) ensured that the water splitting reaction could take place with no external bias and separate H2 and O2 evolution could be achieved. The destructi...

Effects of phosphorylation of bovine cardiac troponin T (TnT) by protein kinase C on the Ca(2+)-stimulated MgATPase activity of reconstituted actomyosin complex and the binding of TnT to tropomyosin(Tm)-F-actin were investigated. The Ca(2+)-stimulated MgATPase of actomyosin containing phosphorylated TnT (1.8 mol of P/mol), compared with that containing unphosphorylated TnT, was decreased by up ...