The clinical signs observed in H. canis-infected dogs in Brazil were anorexia, pale mucous membranes, weight loss, diarrhoea, gait abnormalities, fever, polyuria and polydipsia. The laboratory findings were anemia, leucocytosis with neutrophilia, lymphopenia, monocytosis, and elevated alkaline phosphatase (Gondim et al., 1998; Paludo et al., 2003; Aguiar et al., 2004). All the animals in these ...

The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that ...

conclusions our findings showed that streptomyces has antifungal effects against m. canis. results in this study the most antagonist of actinomycete isolates with antifungal activity against m. canis isolates of l1, d5, ks1m, km2, kn1, ks8 and ks1 were shown in vitro. electron microscopic studies showed that some fungal strains form spores, mycelia and spore chain. nucleotide analysis showed th...

Giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of Babesia canis and Ehrlichia canis. Since Cowdria ruminantium cross-reacts with Ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (IFA) test in which C. ruminantium was...

Using STAT6(-/-) BALB/c mice, we have analyzed the role of STAT6-induced Th2 response in determining the outcome of experimental toxocariasis caused by embryonated eggs of the helminth parasite Toxocara canis. Following T. canis infection wild-type BALB/c mice developed a strong Th2-like response, produced high levels of IgG1, IgE, and IL-4, recruited alternatively activated macrophages, and di...

BACKGROUND Phellodendron amurense, exhibits antifungal activity mainly by bioactive components including berberine hydrochloride and palmatine hydrochloride. This study was conducted to evaluate the antifungal effects of berberine hydrochloride, palmatine hydrochloride, and a mixture of both substances against Microsporum canis in vivo and in vitro. METHODS The minimal inhibitory concentratio...

A cell wall antigen of Brucella canis was purified by immunosorbent columns. The antigen contained two proteins of 30 and 28 kilodaltons and a polysaccharide exhibiting a 12-kilodalton band upon 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antibody to the purified antigen, which specifically reacted with the polysaccharide, was used as the first coating antibody in an enzyme...

Human serological assays designed to detect brucellosis will miss infections caused by Brucella canis, and low levels of periodic bacteremia limit diagnosis by blood culture. Recent B. canis outbreaks in dogs and concomitant illnesses in caretakers suggest that unapparent human infections may be occurring. With more than a quarter of a million persons in occupations involving dogs, and nearly 8...

ing captures information about the cable itself: its document number, source, date, etc. Indexing captures information about the entities described in the cable: their names, dates of birth, locations, etc. The result of the abstracting and indexing process is a set of index records about the entities that were described in a cable. When a cable has been abstracted and indexed, its index record...

Diarrhea caused by intestinal coccidia (Cystoisospora species) is a common problem in pet dogs and in dogs in animal shelters. Cystoisospora canis has the largest oocysts of the 4 named species of coccidia infecting dogs. The present study examined an isolate of C. canis obtained from a dog from São Paulo, SP, Brazil. Oocysts sporulated within 2 days at room temperature, and 20 sporulated oocys...