Biogenesis of mammalian mitochondrial ribosomes requires a concerted maturation of both the small (SSU) and large subunit (LSU). We demonstrate here that the m(5)C methyltransferase NSUN4, which forms a complex with MTERF4, is essential in mitochondrial ribosomal biogenesis as mitochondrial translation is abolished in conditional Nsun4 mouse knockouts. Deep sequencing of bisulfite-treated RNA s...

We have identified previously in mitochondrial DNA of the colorless, chlorophycean, green algal taxon, Polytomella parva, potential coding regions for four small subunit (SSU) and eight large subunit (LSU) rRNA fragments. In this study with P.parva, we isolated RNA from a mitochondrial-enriched preparation, characterized the 12 mitochondrial rRNA transcripts by either northern blot analysis or ...

Curcumae Longae Rhizoma (Curcuma longa L.) is an important traditional Chinese medicine with multiple beneficial effects. To elucidate the genetic and chemical differences among Curcumae Longae Rhizoma samples, three DNA barcoding markers (rbcL, matK, and ITS-LSU D1/D3) and HPLC fingerprinting were employed in this study. The discriminatory power of rbcL and matK was low, as they only detected ...

Land degradation and poverty caused by traditional arable agriculture have been serious problems in the ecologically fragile and economically backward mountains of Nepal. There is a prospect to address both of these problems to some extent by promoting livestock raising. However, a large livestock population exceeding the carrying capacity of land resources will risk undermining the sustainabil...

Eleanor Daveron, M.D.,∗ Neeraj Jain, M.D.,∗ Glenn P. Kelley, M.D.,∗ William H. Luer, M.D.,∗∗ Cesar Fermin, Ph.D.,∗∗ Frederick Helmcke, M.D.,∗ and Edmund K. Kerut, M.D.†,‡ ∗Division of Cardiology, LSU Health Sciences Center, New Orleans, Louisiana, ∗∗Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, Louisiana, †Departments of Pharmacology and Phy...

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity...

Rickettsia felis (Alphaproteobacteria: Rickettsiales) is the causative agent of an emerging flea-borne rickettsiosis with worldwide occurrence. Originally described from the cat flea, Ctenocephalides felis, recent reports have identified R. felis from other flea species, as well as other insects and ticks. This diverse host range for R. felis may indicate an underlying genetic variability assoc...