Micropropagation of banana (Musa paradisiaca Linn.) var Grand naine was carried out with media sterilized by microwave radiation. Microwave oven was effectively used to sterilize the media for in vitro shoot-multiplication of banana against autoclave. Sterilization of media in microwave oven was successfully achieved at 900W/200 ml media (4 min). In vitro shoot-multiplication was higher in the ...

Canna indica Linn. (Cannaceae) is used both as medicine and food. Traditionally, various parts of C. indica are exploited to treat blood pressure, dropsy, fever, inflammatory diseases etc. However, to date there is no reliable micropropagation protocol for C. indica. We present here a regeneration technique C. indica with banana micropropagation medium (BM). BM supplemented with 3% sucrose, 0.7...

The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerate...

One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied ...

The use of micropropagated trees has been widely extended, since micropropagation is a convenient technique of vegetative propagation that shows a number of interesting advantages, mainly for woody fruit trees. However, only few studies can be found in the literature on the field performance of micropropagated trees. In this work, the performance of different micropropagated fruit-tree rootstoc...

In vitro micropropagation of wild cherry is presently one of the main commercial ways to clonally propagate this species (Cornu and Boulay, 1986). In order to extend this technique to a large number of clones, it seems necessary to improve our knowledge of the behavior of the explants during the in vitro culture. Since plant growth regulators (PGR) play an important role in this technique (Marg...

Introduction: Squirting cucumber, Ecballium elaterium (L.) A. Rich. (Cucurbitaceae), is a wild medicinal plant found abundantly in the Mediterranean region. It has been utilized as a rootstock for many cucurbitaceous crops, mainly attributed to its resistance to abiotic as well as biotic stress (2). Important pharmacological uses (1, 9) are attributed to the bitter principles, cucurbitacins (5)...

Plants have been used for medicinal application ever since man has begun caring for his body and health. For centuries, the world has depended on the valuable properties of plant as a source of healing. Based on the considerable knowledge about these plant species from the ancient books, the modern systems of medicine have enabled these plants to find a place in the commercial MIcroProPAGAtIon ...

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm-3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm-3). Cultures were maintained in darkness. Soaking explants ...

A protocol for in vitro cloning of mature plants of Citrus limon using nodal shoot segments has been developed. Three to four shoots were induced per axillary meristems on MS medium amended with 9 μM BAP. Repeated transfer of the initial explants for up to six passages on MS medium [having 50% of the original concentrations of NH4NO3 and KNO3, and supplement of 250 mg L (NH4)2SO4] with 0.22 μM ...