Laser capture microdissection (LCM) offers a reliable cell population enrichment tool and has been successfully coupled to MS analysis. Despite this, most proteomic studies employ whole tissue lysate (WTL) analysis in the discovery of disease biomarkers and in profiling analyses. Furthermore, the influence of tissue heterogeneity in WTL analysis, nor its impact in biomarker discovery studies ha...

AUTHORS Vanessa VIEIRA / CICECO AVEIRO INSTITUTE OF MATERIALS, UNIVERSITY OF AVEIRO; ASSOCIATE LABORATORY LSRE-LCM, POLYTECHNIC INSTITUTE OF BRAGANÇA; CIMO MOUNTAIN RESEARCH CENTER, POLYTECHNIC INSTITUTE OF BRAGANÇA, CICECO AVEIRO INSTITUTE OF MATERIALS, DEPARTMENT OF CHEMISTRY, CAMPUS UNIVERSITÁRIO DE SANTAGO, AVEIRO Olga FERREIRA / ASSOCIATE LABORATORY LSRE-LCM, POLYTECHNIC INSTITUTE OF BRAGA...

INTRODUCTION In recent year, the design concepts of spinal implants alternation from traditional stable fusion to restore normal spinal range of motion (ROM). Therefore, the method of the spinal implants in vitro test was advanced from traditional load control method (LCM) to displacement control method (DCM) [1-3]. In real life, it is still not clear whether to use LCM or the DCM to evaluate t...

We describe an approach to optical lithography using light-scattering contact masks with protruding elements that couple light into a photoresist. This method differs from conventional contact lithography in two important ways. First, because portions of the light-coupling mask ~LCM! are made from a polymer, intimate contact with the resist occurs over large areas without additional load. This ...

Laser capture microdissection (LCM) facilitates the isolation of individual cells from tissue sections, and when combined with RNA amplification techniques, it is an extremely powerful tool for examining genome-wide expression profiles in specific cell-types. LCM has been widely used to address various biological questions in both animal and plant systems, however, no attempt has been made so f...

Abstract In multivariate Geostatistics, the linear coregionalization model (LCM) has been widely used over last decades, in order to describe spatial dependence which characterizes two or more variables of interest. However, spatio-temporal multiple modeling, identification main elements a space–time (ST-LCM), as well latent structures underlying analyzed phenomenon, represents tough task. this...

Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have opti...