A mixture of threonine dehydrogenase and aminoacetone synthetase will catalyze the conversion of L-threonine to glycine. The overall reaction likely involves the conversion of L-threonine, NAD+, and CoA to glycine, NADH, and acetyl-CoA. Physical separation of L-threonine dehydrogenase from aminoacetone synthetase results in the formation of aminoacetone and CO2 from their substrates. A physical...

The activities of 4-aminobutyric-2-oxoglutaric acid transaminase (GABA-T) and succinate semialdehyde dehydrogenase (SSADH) were determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-X)and/or caerulein (CLN). GABA transaminase activity was measured utilizing endogenous succinate semialdellyde dehydrogenase to convert the product of GAB A-T, succinate semiald...

Enzymatic reactions can successfully replace complex chemical syntheses using milder reaction conditions and generating less waste. The developed model-based numerical analysis turned out to be a beneficial tool determine the optimal operating policies of multienzymatic reactors. As proved, for such cases, determination Fed-Batch Reactor (FBR) policy results in difficult multiobjective optimiza...

The fluorescence emission intensity at 460 nm of reduced nicotinamide adenine dinucleotide in solution is increased in the presence of deuterium oxide over that observed in water. The increase in fluorescence intensity is a linear function of DzO concentration, and in 75 volume % DzO equals 20%. A similar increase is observed with N-methyldihydronicotinamide, reduced 3-acetylpyridine adenine di...

Since the experiments of KEARNEY 1 it has been known that both particle-bound and soluble succinate dehydrogenase can be activated by its substrate succinate and by competitive inhibitors such as fumarate, malonate and phosphate. Binding at the active centre apparently brings the enzyme in an active conformation. GUTMAN et al. 2) 3 have recently reported that NADH is as effective as succinate i...

Abstract NADH-dependent phenylalanine amine dehydrogenase (F-AmDH) engineered from (PheDH) catalyzes the synthesis of aromatic chiral amines prochiral ketone substrates. However, its low coenzyme affinity and catalytic efficiency limit industrial application. Here, we developed a chimeric dehydrogenase, cFLF-AmDH, based on relative independence structure at domain level, combined with substrate...

Despite the lack of biochemical information, all available in silico metabolic models of Pseudomonas putida KT2440 consider NADP as the only cofactor accepted by the glucose-6-phosphate dehydrogenases. Because the Entner-Doudoroff pathway is the main glycolytic route in this bacterium, determining how much NADH and NADPH are produced in the reaction catalyzed by these enzymes is very important ...

1. Cystamine was reduced to the corresponding thiol by rat liver mitochondria, even in the presence of rotenone or antimycin A. 2. The reduction of disulphides was stimulated by the accumulation of NADH or by the addition of NADH to osmotically ;shocked' mitochondria. 3. Energy made available by oxidative phosphorylation was not essential for the reduction of disulphides. 4. Cystamine was not r...

Background: Globally 10-15% couples suffer from infertility and in about 50% of these cases the problem is attributed to male partner. One of the major genetic cause of male factor infertility is point mutations in mtDNA. Methods: In the present study a systematic sequence analysis of 6 mitochondrial genes (NADH dehydrogenase 2, NADH dehydrogenase 3, NADH dehydrogenase 4L, NADH dehydrogenase 4,...

EVIDENCE FOR TWO SITES OF SUPEROXIDE PRODUCTION BY MITOCHONDRIAL NADH-Q OXIDOREDUCTASE (COMPLEX I)* Jason R. Treberg, Casey L. Quinlan and Martin D. Brand Buck Institute for Research on Aging, Novato, CA, 94945, USA Running head: Two sites of superoxide production by complex I Address correspondence to: Jason R. Treberg, 8001 Redwood Blvd., Novato, CA, 94945. FAX: 415 209 2232. Email: jtreberg@...