Orchidaceae is the second largest family of flowering plants, which is highly valued for its ornamental purposes and medicinal uses. Dendrobium officinale is a special orchid species that can grow without seed vernalization. Because the whole-genome sequence of D. officinale is publicly available, this species is poised to become a convenient research model for the evolutionary, developmental, ...

Allocasuarina verticillata is an actinorhizal tree that lives in symbiotic association with a nitrogen fixing actinomycete called Frankia. In the search for promoters that drive strong constitutive expression in this tropical tree, we studied the organ specificity of four different constitutive promoters (CaMV 35S, e35S, e35S-4ocs and UBQ1 from Arabidopsis thaliana) in stably transformed A. ver...

We developed a multiplex polymerase chain reaction (PCR) coupled with ligation detection reaction and universal array system for screening genetically modified rice and further identifying specific transgenes in rice. Three common elements in transgenic rice (CaMV 35S promoter, FMV 35S promoter and nos terminator) with species and internal controls (rice actin, 18S rRNA) were used for screening...

Cotton leaf curl Burewala virus (CLCuBuV), belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP) genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV) were fused with β-glucuronidase (GUS) and green f...

Nitric oxide (NO) plays a role in defence against hemibiotrophic pathogens mediated by salicylate (SA) and also necrotrophic pathogens influenced by jasmonate/ethylene (JA/Et). This study examined how NO-oxidizing haemoglobins (Hb) encoded by GLB1, GLB2, and GLB3 in Arabidopsis could influence both defence pathways. The impact of Hb on responses to the hemibiotrophic Pseudomonas syringae pathov...

SUMMARY Gene expression is controlled and regulated by interactions between cis ‐regulatory DNA elements (CREs) regulatory proteins. Enhancers are one of the most important classes CREs in eukaryotes. Eukaryotic genes, especially those related to development or responses environmental cues, often multiple enhancers different tissues and/or at developmental stages. Remarkably, little known about...

Plants respond to heat stress by producing heat-shock proteins. These are regulated promoters containing regulatory elements, which can be harnessed control protein expression both temporally and spatially. In this study, we designed heat-inducible produce the diterpene casbene in Nicotiana benthamiana, through a multi-step metabolic pathway. To potentially increase gene transcription, coupled ...

Cauliflower mosaic virus 35S promoter, widely used in transgenic crop plants, is known to be recognized in widely differing kinds of cells. Its activity in human cells may have impact on the risk assessment for the environmental release of genetically modified plants. In this study, transient expression of several constructs containing beta-glucuronidase (GUS) gene driven by cauliflower mosaic ...