Serratia marcescens, a chitinase-producing microorganism, was shown to produce five unique chitinolytic proteins with subunit molecular masses of 21, 36, 48, 52, and 57 kilodaltons. A cosmid library of S. marcescens DNA was constructed in the broad-host-range cosmid pLAFR1 and screened in Escherichia coli for clones capable of degrading chitin. A total of four independent clones (22- to 27-kilo...

Chitinase is necessary for fungal growth and cell division and, therefore, is an ideal target for the design of inhibitors which may act as antifungal agents. A chitinase from the fungal pathogen Coccidioides immitis has been expressed as a fusion protein with gluathione-S-transferase (GST), which aids in purification. After cleavage from GST, chitinase was crystallized from 30% PEG 4000 in 0. ...

Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...

fusarium head blight (fhb) caused by fusarium graminearum is responsible for billions of dollars in agriculture losses. the goal of the present study was evaluation the expression of acidic chitinase, one of pr proteins, in wheat defense response against different fhb induced treatments in 'falat' as a highly susceptible and 'sumai3' as a tolerant cultivar. these treatments contained fungi extr...

Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under...

Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Streptomyces sp. M-20 and purified by ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and Sephadex G-100 gel filtration. No exochitinase activity was found in the culture filtrate. The molecular mass of the purified chitinase was 20 kDa, estimated by a sodium dodecyl sulfate-polyacrylamide gel electrop...

A two-molecule chitinolytic enzyme system (endoand exo-chitinase) hydrolyzes and degrades the chitin polymers. Therefore, it is imperative to discover novel compounds for inhibiting chitinolytic enzymes to prevent insect growth. This research examined the effect of pentoxifylline (a dimethylxanthine chitinase inhibitor) on inhibition of endoand exo-chitinolytic enzyme activities in eastern subt...

The temporal and spatial expression of a bean chitinase promoter has been investigated in response to fungal attack. Analysis of transgenic tobacco plants containing a chimeric gene composed of a 1.7-kilobase fragment carrying the chitinase 5B gene promoter fused to the coding region of the gus A gene indicated that the chitinase promoter is activated during attack by the fungal pathogens Botry...

ABSTRACT: Streptomyces sampsonii is a kind of biocontrol bacterium with antifungal effects, and chitinase one the main substances. To improve further study structure function gene S. sampsonii, we amplified target fragment by PCR, ligated to expression vector pET-32a, introduced resulting plasmid into Escherichia coli BL21 (DE3) induced chitinase. Then, recombinant was purified is-labelled prot...

MOTIVATION Multiple chitinases as well as lectins closely related to them have been characterized previously from many insect species and the corresponding genes/cDNAs have been cloned. However, the identification of the entire assortment of genes for chitinase family proteins and their differences in biochemical properties have not been carried out in any individual insect species. The complet...