Escherichia coli, an opportunistic environmental pathogen, is one of the commonest causes of bovine clinical mastitis in the early lactation period. Shiga toxin-producing E. coli (STEC) whose pathogenesis is associated with enterotoxigenic genes (stxI, stxII, and eaeA) cause serious acute illness and long-term side effects in both animals and humans. The aim of this study was to investigate the...

In a previous study, enterohemorrhagic Escherichia coli (EHEC) O157:H7 with a deletion and insertion in the eaeA gene encoding intimin was used to establish that intimin is required for the organism to attach to and efface microvilli in the piglet intestine (M. S. Donnenberg, S. Tzipori, M. L. McKee, A. D. O'Brien, J. Alroy, and J. B. Kaper, J. Clin. Invest. 92:1418-1424, 1993). However, in the...

Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains are clinically significant food-borne pathogens. However, there is a dearth of information on serotype prevalence and virulence gene distribution, data essential for the development of public health protection monitoring and control activities for the meat and dairy industries. Thus, the objective of this study was to examine the pr...

Diarrheal diseases can lead to infections and cause morbidity mortality in children. Diarrheagenic Escherichia coli (DEC) is an etiological agent, which considered the major causative agent of diarrhea children some developing countries. The aims this work were estimate (E. coli) causing less than 5 years old, detect biofilm virulence factors effect antibiotics. For methodology, a total 112 spe...

The presence of sorbitol-negative Escherichia coli O157 was investigated in healthy Awassi sheep faeces from 175 randomly selected animals in Burdur province of Turkey. Out of 175 animals, 16 (9.1%) were faecal shedding of sorbitol-negative E. coli O157. Out of the 15 flocks included in the study, 7 (47%) had at least one sheep positive for sorbitol-negative E. coli O157. The isolation rate of ...

AIM Aim of the study was to investigate the prevalence, virulence gene profiles, and antimicrobial resistance pattern of Shiga toxigenic Escherichia coli (STEC) in diarrheic buffalo calves from Andhra Pradesh and Telangana States. MATERIALS AND METHODS A total of 375 fecal samples from diarrheic buffalo calves of 1-7, 8-30, 31-60, and 61-90 days age were collected from which STEC were isolate...

The aim of this study was to detect the virulence factors, serogroups, and antibiotic resistance properties of Shiga toxin-producing Escherichia coli, by using 268 bovine mastitic milk samples which were diagnosed using California Mastitis Test. After E. coli identification, PCR assays were developed for detection of different virulence genes, serogroups, and antibiotic resistance genes of Esch...

background and objectives: shiga toxin-producing escherichia coli ( stec) have emerged as human pathogens and contamination of foods of animal origin has been a major public health concern. the aim of the present study was to determine the dissemination of stec in healthy and diarrheic calves in urmia region which is located in west azerbaijan province, iran. materials and methods: in the curre...

objective: the aim of the this study was to investigate the prevalence of toxb, paa, lpf and iha adhesion genes in enteropathogenic escherichia coli (epec) isolates lacking in two important adhesion factors, the eaea and bfpa genes. methods: we examined a total of 70 serologically confirmed epec (eaea-, bfpa-) isolates. dna from the isolates was extracted by the phenol-chloroform method. toxb,...

The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them posse...