Zinc-α2-glycoprotein (ZAG) plays an important role in the regulation of body weight, body fat, and glucose metabolism. In this study, we first measured ZAG levels in serum and ZAG mRNA levels in subcutaneous white adipose tissue (sWAT) among overweight/obese patients and lean control subjects. Second, we investigated the effects of ZAG administration on the body weight, body fat and glucose met...

1 Detrimental In Pressure Overloaded Mouse Hearts 2 3 Georgios Karamanlidis*, Lorena Garcia-Menendez*, Stephen C. Kolwicz, Jr., Chi Fung 4 Lee, Rong Tian. 5 Mitochondria and Metabolism Center, Department of Anesthesiology & Pain Medicine, 6 University of Washington, Seattle, Washington, USA. 7 Running title: PGC1α overexpression in cardiac pressure overload 8 9 Corresponding author: 10 Rong Tia...

PURPOSE Chemotherapy treatment of metastatic colon cancer ultimately fails due to development of drug resistance. Identification of chemotherapy-induced changes in tumor biology may provide insight into drug resistance mechanisms. EXPERIMENTAL DESIGN We studied gene expression differences between groups of liver metastases that were exposed to preoperative chemotherapy or not. Multiple patien...

Chronic chagasic cardiomyopathy (CCM) is presented by increased oxidative/inflammatory stress and decreased mitochondrial bioenergetics. SIRT1 senses the redox changes and integrates mitochondrial metabolism and inflammation; and SIRT1 deficiency may be a major determinant in CCM. To test this, C57BL/6 mice were infected with Trypanosoma cruzi (Tc), treated with SIRT1 agonists (resveratrol or S...

Inhibiting class I histone deacetylases (HDACs) increases energy expenditure, reduces adiposity and improves insulin sensitivity in obese mice. However, the precise mechanism is poorly understood. Here, we demonstrate that HDAC1 is a negative regulator of brown adipocyte thermogenic program. HDAC1 level is lower in mouse brown fat (BAT) than white fat, is suppressed in mouse BAT during cold exp...

Leucine supplementation has been shown to prevent high-fat diet (HFD)-induced obesity, hyperglycemia, and dyslipidemia in animal models, but the underlying mechanisms are not fully understood. Recent studies suggest that activation of Sirtuin 1 (SIRT1) is an important mechanism to maintain energy and metabolic homeostasis. We therefore examined the involvement of SIRT1 in leucine supplementatio...

Oxygen sensing is associated with mitochondrial function. EglN2, which contributes to breast tumorigenesis as a prolyl hydroxylase, has recently been identified as a transcription co-activator through interaction with NRF1 and PGC1α to regulate mitochondrial function under conditions of normoxia and hypoxia. FDXR is the important downstream target gene that mediates this regulation.