Leukotriene A4 synthase was purified from the cytosolic fraction of murine mast cells. The enzyme converted 5-hydroperoxy-6-trans-8,11,14-cis-icosatetraenoic acid (5-HPETE) to leukotriene A4. This unstable product was identified by demonstration of two epimers of 6-transleukotriene B4, methanol trapping, as well as further transformation to leukotriene B4 by leukotriene A4 hydrolase. Leukotrien...

In this paper, we determine the homotopy groups π4(ΣK(A, 1)) and π5(ΣK(A, 1)) for abelian groups A by using the following methods from group theory and homotopy theory: derived functors, the Carlsson simplicial construction, the Baues-Goerss spectral sequence, homotopy decompositions and the methods of algebraic K-theory. As the applications, we also determine πi(ΣK(G, 1)) with i = 4, 5 for som...

The nal¨ve library from the lymphocytes of healthy humans was screened by murine single-stranded idiotypic antibodies against benzo[a]pyrene (pSh). The phage clone which contained of anti-idiotypic antibody against benzo[a]pyrene, designated as A4, was chosen for further work because of highly specific to pSh. The available protein databases were searched. The A4 amino acid sequence was unique ...

A variety of melanoma antigen A (MAGE-A) genes are commonly detected in non-small cell lung cancers. Their biological function is not well characterized but may involve the regulation of apoptosis and cell cycle progression. We hypothesized that MAGE-A4 is involved in the regulation of apoptosis. To investigate this, expression of MAGE-A was evaluated. MAGE-A4 was expressed in 48% of non-small ...

argumentation frameworks [Dun95] take a very simple view on argumentation as they do not presuppose any internal structure of an argument. Abstract argumentation frameworks only consider the interactions of arguments by means of an attack relation between arguments. Definition 1 (Abstract Argumentation Framework). An abstract argumentation framework AF is a tuple AF = (Arg,→) where Arg is a set...

A4 Simulator is a modeling and simulation environment to aid the performance optimisation of the service advertisement and discovery in the practical A4 system. A4 Simulator supports all kinds of the performance optimisation strategies and can output several kinds of simulation results on different high-performance criterions. It also supports the service reconfiguration for the agent mobility....

Grafting desirable scion on stress-tolerant rootstocks provides an opportunity to improve the cryotolerance of scion. Genic male sterile (GMS) lines of plant could be used as sterile line and maintainer in breeding, and they have the conspicuous characteristics that the fertility of which is easy to regain but hard to maintain by sexual reproduction. In order to maintain the fertility of GMS co...

argumentation frameworks [Dun95] take a very simple view on argumentation as they do not presuppose any internal structure of an argument. Abstract argumentation frameworks only consider the interactions of arguments by means of an attack relation between arguments. Definition 1 (Abstract Argumentation Framework). An abstract argumentation framework AF is a tuple AF = (Arg,→) where Arg is a set...

Trans-lesion synthesis (TLS) is an important DNA-damage tolerance mechanism that permits ongoing DNA synthesis in cells harbouring damaged genomes. The E3 ubiquitin ligase RAD18 activates TLS by promoting recruitment of Y-family DNA polymerases to sites of DNA-damage-induced replication fork stalling. Here we identify the cancer/testes antigen melanoma antigen-A4 (MAGE-A4) as a tumour cell-spec...

We describe a novel fragment library termed fragments of life (FOL) for structure-based drug discovery. The FOL library includes natural small molecules of life, derivatives thereof, and biaryl protein architecture mimetics. The choice of fragments facilitates the interrogation of protein active sites, allosteric binding sites, and protein-protein interaction surfaces for fragment binding. We s...