نتایج جستجو برای: 14c

تعداد نتایج: 10343  

Journal: :Clinical chemistry 1986
R N Johnson J R Baker

We investigated the ability of the fructosamine assay to detect glycation of serum proteins. We incubated both whole human serum and serum protein fractions in vitro with [14C]glucose, and analyzed for reducing activity and for uptake of 14C by protein. In all experiments, the reducing activity increased linearly with time for seven days and was correlated with 14C uptake (r = 0.94-0.98). Prote...

Journal: :The Biochemical journal 1977
Z Kiss

Sphingomyelin synthesis was studied in slices of rat heart by using [Me-14C]choline, [1,2-14C]ethanolamine, S-adenosyl-L-[14C]methionine and [32P]Pi as as precursors. In the presence of both [Me-14C]choline and [32P]Pi the ratio of the specific radioactivities of 14C and 32P in phosphatidylcholine was greater than in sphingomyelin at all the times studied. This suggested that synthesis of phosp...

Journal: :The Journal of biological chemistry 1981
M Yoshida J W Poser W S Allison F S Esch

The TF1-ATPase from the thermophilic bacterium, PS3, is inactivated by dicyclohexylcarbodiimide (DCCD). This inactivation is stimulated by ADP and other specific nucleotides and is inhibited by Mg2+. When the inactivation is carried out with [14C]DCCD, about 2 g atoms of 14C are bound/mol of TF1 when the enzyme is nearly completely inactivated. The isolated subunits from TF1 inactivated with [1...

Journal: :Applied and environmental microbiology 1993
I P Armstead J R Ling

Mixed ruminal bacteria, isolated from sheep (Q and W) fed a concentrate and hay diet, were anaerobically incubated with either 14C-peptides or 14C-amino acids. Experiment 1 showed that uptake of both 14C-labeled substrates was rapid, but the rate for amino acids was twofold greater than for peptides (molecular weight, 1,000 to 200) initially but was similar after 10 min. Experiment 2 demonstrat...

Journal: :The Biochemical journal 1984
L M Roeder J T Tildon D C Holman

The rates of conversion of D-(-)-3-hydroxy[3-14C]butyrate, [3-14C]acetoacetate, [6-14C]glucose and [U-14C]glutamine into 14CO2 were measured in the presence and absence of alternative oxidizable substrates in intact dissociated cells from the brains of young and adult rats. When unlabelled glutamine was added to [6-14C]glucose or unlabelled glucose was added to [U-14C]glutamine, the rate of 14C...

Journal: :Bioscience reports 1991
N Carbó F J López-Soriano J M Argilés

Hepatocytes isolated from obese Zucker rats showed a significantly higher rate of both [U-14C]glucose and [U-14C]lactate incorporation into [14C]lipid than those from their lean counterparts. This was associated with a marked increase in the lipogenic rate measured by the incorporation of 3H2O into the cell esterified fatty acids. Although there were no changes in the incorporation of the trace...

Journal: :The Journal of Cell Biology 1968
Robert M. Kodama Henry Tedeschi

A study of the permeability of calf thymus nuclei isolated in sucrose was carried out with sucrose-14C, glycerol-14C, and carboxydextran-14C (molecular weight, 60,000-90,000). The results indicate that the nuclei are very permeable to both sucrose and glycerol but they exclude the carboxydextran. Results obtained with other low molecular weight non-electrolytes (malonamide-14C, erythritol-14C, ...

Journal: :British journal of industrial medicine 1993
Y Katakura R Kishi T Okui T Ikeda H Miyake

The distribution of 14C after the administration of 14C-formaldehyde was studied in pregnant mice by a whole body low temperature autoradiographic technique. The concentrations of formaldehyde and its metabolites in maternal and fetal blood and tissues were determined in unsectioned tissues by liquid scintillation spectrophotometry. The binding of 14C from 14C-formaldehyde to cells and DNA in m...

Journal: :International Biodeterioration & Biodegradation 2021

Abstract This study investigated the extractability, bioaccessibility and biodegradation of 14C-phenanthrene 14C-octacosane in two soils from former oil refinery facilities over 341 days. The impact biostimulation bioaugmentation treatments was also evaluated. At 0, 31, 62, 124 days, loss extractability (using dichloromethane, methanol:water hydroxypropyl-?-cyclodextrin (HPCD)) 14C-hydrocarbons...

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