Rapid initiation of antibiotic treatment and fast diagnosis are essential in bacterial infection of the central nervous system (CNS). Culture as common method for detecting bacteria is time consuming and unreliable once antibiotic treatment has been initiated. Eubacterial 16S rDNA-PCR with species differentiation by sequencing appears to be a promising tool. Our experiences with this method per...

background and objectives: anaplasma phagocytophilum is a zoonotic, tick borne rickettsial pathogen. a. phagocytophilum has been detected in north america, europe, africa and asia by molecular methods. in iran we have little information about the distribution of this agent in human and animals. materials and methods: from march 2007 to july 2007, one hundred and fifty blood samples and correspo...

background: a. bovis and a. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. they are obligate intracellular bacteria that can infect and cause anaplasmosis in human and animals. therefore, this study was carried out to detect a. bovis and a. phagocytophilum in naturally infected dairy cattle in isfahan using molecular techniques. materials and methods: in this study a total ...

pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. a polymerase chain reaction (pcr) assay was developed using primers derived from conserved part of 16s-23s rrna gene. the pcr amplified a fragment size of 0.7 kb using dna from nine avian p. multocida  isolates...