RNase P preparations from Escherichia coli can be separated into RNA and protein by chromatography, in buffers containing 7 M urea, on Sephadex G-200, DEAE-Sephadex, or CM-Sephadex columns. Neither RNA nor protein components alone exhibits any RNase activity. RNase P activity can be reconstituted by mixing separated RNA and protein components in buffer containing 7M urea followed by dialysis of...

Independently folded domains in RNAs frequently adopt identical tertiary structures regardless of whether they are in isolation or are part of larger RNA molecules. This is exemplified by the P15 domain in the RNA subunit (RPR) of the universally conserved endoribonuclease P, which is involved in the processing of tRNA precursors. One of its domains, encompassing the P15 loop, binds to the 3'-e...

As compared to whole milk feeding, replacer is an excellent cheaper source of nutrition for calves prior weaning. For this study, 20 newborn HF crossbred were divided into two equal groups each 10 animals and put under 8 weeks feeding trial. The control group (T1) fed cow’s as per owner’s practice in the field, while those treatment (T2) @ 2.5 ltr during 1st week then (Amul brand) was 50 gm 150...

Na colheita mecanizada de cana-de-açúcar, espessas camadas palha são depositadas sobre o solo dificultando a rebrota e emergência Saccharum sp. Faz-se necessário buscar alternativas para seu excedente, que pode ser destinado à produção biochar, material rico em carbono atua como condicionador solos sequestrador CO2 da atmosfera. O objetivo foi produzir biochar utilizando resíduos cana, analisan...

Cellgevity® is a supplement reported to comprise mostly D-Ribose and L-Cysteine enriched glutathione, known be an effective antioxidant that improves spermatozoa quality. However, its effect on milt characteristics has not been reported. This study, therefore, aimed evaluate the effects of quality catfish (Clarias gariepinus) extended in sodium citrate during chilled storage. Pooled sample from...