In this Article, the legend of Figure 5 and Figure 6 are incorrect: In Figure 5: " Phylogenetic analysis of RPSA in insect species. Neighbor-joining method was used to construct the phylogenetic tree. Bootstrap values with 1000 trials are indicated on branches. " should read: " Quantification of the expression level of fruitless gene in ovary and testis of P. americana with real-time PCR. The e...

RNA quality is of utmost importance to perform gene expression quantification by qPCR. The classical methods used to determine RNA quality are based on electrophoresis and spectrophotometer assessment, namely A(260)/A(280) and A(260)/A(230) ratios. It was previously shown that due to the complex nature of Staphylococcus epidermidis biofilms, RNA extraction procedures could impact mRNA quality a...

The use of RT-qPCR provides a powerful tool for gene expression studies; however, the proper interpretation of the obtained data is crucially dependent on accurate normalization based on stable reference genes. Recently, strong evidence has been shown indicating that the expression of many commonly used reference genes may vary significantly due to diverse experimental conditions. The isolation...

pregabalin (prg) is a new antiepileptic drug and aceclofenac (ace) is a potent non-steroidal anti-inflammatory drug. these drugs in combination are used for treatment of partial seizures and neuropathic pains. a simple and precise assay method by rp-hplc was developed and validated for estimation of prg and ace in acenac-n tablet. analyses of commercial tablet, acenac-n were performed using jas...

different software tools for quantification of myocardial perfusion spect (mps) studies are routinely used. several perfusion parameters can be computed automatically.  interpretation of the mps should start with visual inspection of the rotating planar images, visual analysis of reconstructed spect slices and then quantitative analysis to confirm the visual impression. quantification should be...

Changes in mitochondrial DNA (mtDNA) content is a useful clinical biomarker for various diseases, however results are controversial as several analytical factors can affect measurement of mtDNA. MtDNA is often quantified by taking ratio between a target mitochondrial gene and a reference nuclear gene (mtDNA/nDNA) using quantitative real time PCR often on two separate experiments. It measures re...

The rapid evolution of techniques for measuring gene expression makes available substantial data which require careful analysis. In particular, relative quantification based on microfluidic cards allows performing of rapid large scale analyses. In the present study, we employed ovarian carcinoma cell lines resistant to cisplatin (IGROV-1/Pt1) or to a camptothecin (IGROV-1CPT/L), both characteri...

Background: The egg yolk precursor protein vitellogenin (VTG) has proven to be a useful biomarker, used to identify organisms exposed to estrogenic compounds. Methods: We investigated variations in the VTG gene expression pattern and plasma sex steroid hormones concentrations in the yellowfin Seabream, Acanthopagrus latus, (A. latus) by various doses of bisphenol-A (BPA) exposure for 7 and 14 ...