The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as substrate was greater than 10% of that with 3-hydroxybutyrat...

The aim of this study was to evaluate the effect of the season (ambient temperature and relative humidity) on some blood metabolites in dairy cows during postpartum period. Blood samples were collected from 195 clinically healthy dairy cows of 8 Italian dairy herds in spring (April-May 15.5±4 °C and 69.5±0.7 %), summer (June-July 23±1.5 °C and 69±1.3 %) and autumn (September-October 17±2 °C and...

1. In liver, rumen epithelium and kidney cortex of the sheep, a dehydrogenase active against dl-3-hydroxybutyrate occurred in both the cytosol and particulate fractions of the tissues. In brain, heart, skeletal and smooth muscles, the enzyme occurred only in the particulate fraction. 2. Enzyme activity in the cytoplasmic fraction of liver and rumen epithelium was similar with either d(-)-3-hydr...

Recent studies have highlighted the therapeutic and ergogenic potential of ketone body ester, (R)-3-hydroxybutyl-(R)-3-hydroxybutyrate. In present work, enzymatic synthesis this biological active compound is reported. The (R)-3-hydroxybutyl-(R)-3-hydroxybutyrate has been produced through transesterification racemic ethyl 3-hydroxybutyrate with (R)-1,3-butanediol by exploiting selectivity Candid...

The increasing incidence of obesity worldwide and its related cardiometabolic complications is an urgent public health problem. While weight gain results from a negative balance between the energy expenditure and calorie intake, recent research has demonstrated that several small organic molecules containing a four-carbon backbone can modulate this balance by favoring energy expenditure, and al...

We report the draft genome sequence of Halomonas sp. strain KM-1, which was isolated in Ikeda City, Osaka, Japan, and which produces the bioplastic poly(3-hydroxybutyrate). The total length of the assembled genome is 4,992,811 bp, and 4,220 coding sequences were predicted within the genome. Genes encoding proteins that are involved in the production and depolymerization of poly(3-hydroxybutyrat...

It is known that ionophoric antibiotics regulate ruminal fermentation, improve the utilization of feed protein, and prevent occurrence ketosis steatosis in ruminants. Ionophoric β-acids hops have a similar spectrum biological activity, is, they inhibit vital activity most gram-positive microorganisms rumen. Bacteria, like other living organisms, need vitamin E as an active antioxidant for cell ...

High cost of poly-3-hydroxybutyrate production remained a major hindrance for its wide range applications. In the current study poly-3-hydroxybutyrate producing bacteria were isolated from environmental sources. Highest poly-3hydroxybutyrate producing isolate Bacillus cereus PS 10 was investigated for its ability to use wide range of low-cost carbon sources including agro-industrial residues, v...