Preparation of Tissue-Studies were performed on the isolated urinary bladder of the toad, Bujo marinus. Animals were pithed and the two halves of the bladder rapidly removed through a ventral incision. The tissue was rinsed in Ringer’s solution before use. For the study of the oxygen consumption of various toad tissues, small intestine, stomach, heart, ventral abdominal skin, and skeletal muscl...

In order to evaluate the inter-specific hybrid of P. atlantica × P. vera cv. ‘Badami Zarand’ to salinity stress, an experiment was conducted as a factorial based in a completely randomized design with 0, 60, 120 mM salinity levels of NaCl2, CaCl2 and MgCl2 (3:2:1) on two rootstocks ‘Qhazvini’ and ‘Badami Zarand’ and an inter- specific hybrid of P. atlantica × P. vera cv. ‘Badami Zarand’. Minera...

in order to investigate the effect of different levels of biological fertilizers on potato performance (cv. marfona), an experiment was carried out as strip plot based on rcb design with 3 replications in 2011 at isfahan. treatments included nitrocara at 4 levels (0: control, 220, 330 and 440 ml.ha-1) as a first factor and nitroxin+ phosphate at 4 levels (0+0: control, 20+100, 30+150 and 50+200...

expansive soils are considered a worldwide problem as they cause extensive damage tocivil engineering structures. an important characteristic of expansive soils is theirsusceptibility to volume change due to the phenomenon of wetting and drying. this paperpresets the effect of pore water quality on the swelling and shrinkage behavior of anexpansive soil during wetting and drying cycles. thirty ...

Distal nephron function of the rat during lithium chloride infusion. Chronic lithium (Li) administration in the rat leads to reduced renal concentration (TCH20) without alterations in renal dilution (CH), To examine the acute effects of lithium on TH2O and C1120, rats were infused with a solution composed of 1% sodium chloride and 1% lithium chloride or a 0.225% lithium chloride solution at rat...

Preparation and crystallization G6-amylase from alkalophilic Bacillus sp.707 was expressed by Bacillus subtilis 207-25. The enzyme was crystallized by the hanging drop vapor diffusion method using the reservoir solution containing 50% (v/v) 2methylpentane-2, 4-diol, 100 mM Tris-HCl (pH 8.5) and 200 mM ammonium phosphate. Calcium chloride and sodium chloride were added to final 1 mM in the drop ...

Mechanisms involved in basolateral H/OH/HCO3 transport in the in vitro microperfused rat cortical thick ascending limb were examined by the microfluorometric determination of cell pH using (2',7')-bis-(carboxyethyl)-(5,6)-carboxyfluorescein. The mean cell pH in this segment perfused with 147 mM sodium and 25 mM HCO3 at pH 7.4 was 7.13±0.02 (n = 30). Lowering bath HCO3 from 25 to 5 mM (constant ...

The distribution and rates of exchange of the ions sodium, potassium, and chloride in single internodal cells of the ecorticate characean, Nitellopsis obtusa, have been studied. In tracer experiments three kinetic compartments were found, the outermost "free space" of the cell, a compartment we have called "protoplasmic non-free space", and the cell sap. The concentrations in the vacuole were 5...

Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mob...