Hepatitis A-virus was purified from human stools by three purification steps. Virus was identified by radioimmunoassay and purity monitored with immune electron microscopy. Virus particles, serologically and morphologically identical, banded in CsCl in two density ranges at 1.31-1.34 g/cm3 and at 1.41-1.43 g/cm3. Virions of density 1.31-1.34 g/cm3 were shown to contain single-stranded DNA of di...

Particles from green and white tissue cultures of Nicotiana tabacum were fractionated by differential and density gradient centrifugation. Besides mitochondria, microbodies and (broken) chloroplasts particles were found able to metabolize 5-amino-levulinate to porphobilinogen and porphobilinogen to uroporphyrinogen. Succinyl-CoA was not synthetized by them but by mitochondria. The porphyrin for...

1,1mg I plus 0,42 mg DL-Phenylalanin-(l-14C) wur­ den in 15 cm3 Wasser gelöst. 1.22 mg II wurden in 15 cm3 Wasser mit 0,5 cm3 7i/lOO-NaOH in das wasser­ lösliche Mono-Natriumsalz überführt und 0,42 mg d l Phenylalanin-(l-14C) zugefügt. Versuchsdauer 48 Stun­ den. Die Isolierung und Reinigung der Isoflavone er­ folgte wie im vorigen Versuch. Ausbeuten: Versuch 1. 4,8 mg Biochanin-A und 1,88 mg F...

Background: The dosimetric parameters required in lung cancer radiation therapy are taken from a homogeneous water phantom; however, during treatment, the expected results are being affected because of its inhomogeneity. Therefore, it becomes necessary to quantify these deviations.Objective: The present study has been undertaken to find out inter- and intra- lung density variations and its dosi...

OBJECTIVE To determine the imaging and demographic characteristics of intracranial haemorrhages, which are subsequently found to be due to an underlying intracranial vascular malformation (IVM). METHODS We compared the demographic and brain imaging characteristics of adults presenting with intracranial haemorrhage, subsequently found to be due to a brain arteriovenous malformation (BAVM), dur...

BACKGROUND The main indication for carotid endarterectomy (CEA) is severity of carotid artery stenosis, even though most strokes in carotid disease are embolic. The relationship between carotid plaque volume (CPV) and symptoms of cerebral ischaemia, and the measurement of CPV by minimally invasive tomographic ultrasound imaging, were investigated. METHODS The volume of the endarterectomy spec...

Left ventricular (LV) volume determinations by the area-length method were reevaluated in postmortem studies of left ventricles ranging from 0.5 to 90 cm3 absolute volume. The regression equation relating known and calculated volumes for calculated volumes <15 cm3 (V' 0.733V) was found to be significantly different from that for calculated volumes >15 cm3 (V'= 0.974V -3.1). From these equations...

Depending on solution conditions, beta-lactoglobulin can exist in one of its six pH-dependent structural states. We have characterized the acid and basic-induced conformational transitions between these structural states over the pH range of pH 1 to pH 13. To this end, we have employed high-precision ultrasonic and densimetric measurements coupled with fluorescence and CD spectroscopic data. Ou...