5'-Methylthioadenosine phosphorylase was purified approx. 340-fold from human prostate by using affinity chromatography by Hg-coupled Sepharose. The enzyme, responsible for the breakdown of 5'-methylthioadenosine into adenine and methylthioribose 1-phosphate, was partially characterized. The apparent Km for 5'-methylthioadenosine is 25 microM. It is activated by thiols and shows an absolute req...

The dripping faucet experiment seeks to provide a relatively simple visualization of a system that exhibits seemingly predictable dynamics that quickly transitions to an entirely unpredictable, chaotic behavior under certain conditions. This behavior is first modeled through equations determine from previosu research and papers in order to understand the general phenomenon and established a bas...

A pathway resulting in the formation of methionine from the 5'-methylthioadenosine is shown in vitro using cell-free homogenates of rat liver. Under the conditions used, methionine was the major product produced, as determined by its chemical and chromatographic properties. The kinetics of product formation indicated that 5'-methylthioadenosine is first rapidly converted to 5-methylthioribose 1...

The pyrimidine, 5-methylcytosine, was demonstrated by Wyatt (1) to be a component of plant and animal deoxyribonucleic acid.’ The deoxyriboside of 5-methylcytosine was isolated from wheat germ DNA by Dekker and Elmore (a), who also characterized this nucleoside. No microorganisms or viruses have yet been shown to contain this base, although the nucleic acids of many of these organisms have been...