the engineering of transgenic canola (brassica napus l. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. glyphosate inhibits the enzyme epsps (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...

Glycine oxidase from Bacillus subtilis is a homotetrameric flavoprotein of great potential biotechnological use because it catalyzes the oxidative deamination of various amines and d-isomer of amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Glyphosate (N-phosphonomethylglycine), a broad spectrum herbicide, is an interesting synthetic amino acid: th...

Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvate (PEP) to L-tyrosine on two plasmids. Rational engineering to improve L-tyros...

A enzyme inhibitors used in agriculture, glyphosate (N-phosphomethyl glycine) is remarkable. A nonselective herbicide discovered in 1970 by a group of scientists at Monsanto led by Dr. John Franz (1), glyphosate, since first commercialization under the trade name Roundup, has been used globally as a safe and effective means of weed control. The discovery of glyphosate’s herbicidal activity was ...

The ectoparasitic dagger nematode (Xiphinema index), vector of Grapevine fanleaf virus (GFLV), provokes gall formation and can cause severe damage to the root system of grapevines. Mycorrhiza formation by Glomus (syn. Rhizophagus) intraradices BEG141 reduced both gall formation on roots of the grapevine rootstock SO4 (Vitis berlandieri×V. riparia) and nematode number in the surrounding soil. Su...

We are studying two enzymes from the shikimate pathway, dehydroquinate synthase (DHQS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Both enzymes have been the subject of numerous studies to elucidate their reaction mechanisms. Crystal structures of DHQS and EPSPS in the presence and absence of substrates, cofactors and/or inhibitors are now available. These structures reveal movemen...

In Nebraska, 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) as well as acetolactate synthase (ALS)-inhibitor-resistant weeds occur in many soybean fields where herbicides from these modes-of-action have been frequently used in the past. Currently, the protoporphyrinogen oxidase (PPO)-inhibitors are the only effective herbicides for POST control of both glyphosateand ALS-inhibitor-resistan...