Determining the immune responses to the development of endometritis during the peripartum period may assist in the development of more efficient reproductive management regimens for dairy herds. In this study, we compared the peripartum immune responses of dairy cows that develop endometritis by 4 weeks postpartum (n = 11) to cows that did not develop this disease (n = 19). Blood samples were c...

Outbreaks of Vaccinia virus (VACV) affecting cattle and humans have been reported in Brazil in the last 15 years, but the origin of outbreaks remains unknown. Although VACV DNA have been already detected in mice (Mus musculus), opossums (Didelphis albiventris) and dogs during VACV zoonotic outbreaks, no transmission to cattle or humans from any of these were reported during Brazilian outbreaks....

background and objectives: leptospirosis is an important zoonotic disease caused by leptospira interrogans . leptospirosis leads to economical losses in dairy farm industry. the objective of this study was to evaluate the pathogenic serovars of leptospira interrogans in dairy cattle herds of shahrekord by pcr. materials and methods: two hundred samples (100 urine and 100 blood) were collected f...

The purpose of this study firstly was conducted to establish a radioimmunoassay (RIA) of estrone sulfate ( E1S ), secondly to monitor the reproductive status of dairy cows using their urine samples. Urine and blood samples were collected in series within a day from four pregnant Holstein-friesian cows to evaluate the relationship between E1S levels in blood and urine with or without urinary cre...

Quality control is essential for any analysis in the laboratory. The objective of this study was to prepare in vivo cow control blood samples. The experiment was performed by feeding cows with a single dose of cadmium in the form of cadmium chloride, withdrawing the blood at an appropriate time to get the highest level of cadmium and detecting the level of cadmium in the blood. It was found tha...

The objectives of this study were to evaluate metabolic and innate immunity alterations in the blood of transition dairy cows before, during, and after diagnosis of lameness during periparturient period. Blood samples were collected from the coccygeal vain once per week before morning feeding from 100 multiparous Holstein dairy cows during -8, -4, disease diagnosis, and +4 weeks (wks) relative ...

During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyzed blood and milk from healthy cows and cows with naturally occurring mastitis...

The objective was to compare insulin resistance parameters in cows with adequate or excessive energy intake as well as in cows with excessive energy intake receiving Cr supplementation as chromium propionate. Thirteen multiparous, nonlactating Gir × Holstein cows were ranked by BW and BCS and assigned to 1 of 3 dietary treatments on d 0: 1) diet to meet their ME requirements without Cr suppleme...

The objectives of this study were to relate concentrations of plasma (PUN) and milk (MUN) urea nitrogen to pregnancy rate in dairy cows and compare various methods of analysis and preparation of milk for measuring MUN. In two experiments, blood or milk samples were collected on the day of AI from Holstein cows (n = 160 and n = 155, respectively). Three methods of MUN analysis were compared. Two...

the amounts of liver dry matter, liver fat and fat in liver dry matter based on age and sex of animals together with the concentrations of glucose, beta hydroxyl butyrate (bhb), urea, triglycerides (tg), calcium magnesium and phosphorus in order to determination of probable subclinical liver lipidosis were investigated in 114 cows including 41 non-pregnant, 7 pregnant, 18 heifers and 48 bulls. ...