A new complex of phleomycin-bleomycin group antibiotics, YA-56, was isolated from the culture broth of a streptomycete designated as strain MCRL 0387 and identified as a new variety of Streptomyces humidus Nakazawaand Shibata in Imamura et al.9 1956. Ultra-violet irradiation of strain MCRL0387 gave a high-yielding mutant which formed blue aerial mycelium instead of the grayish aerial mycelium o...

Recently, mitochondria-targeted photothermal nanoagents demonstrated an improved therapeutic efficacy of cancer cells, compared with non-targeting ones. Herein, copper sulfide (CuS) nanoparticles are in situ synthesized via bovine serum albumin (BSA) templates to prepare BSA@CuS nanocomposites high efficiency (42.0%) conversion. Subsequently, rhodamine-110 (R) molecules covalently conjugated co...

Hypoxic stress is a risk factor of ocular neovascularization. Hypoxia visualization may provide clues regarding the underlying cause of angiogenesis. Recently, we developed a hypoxia-specific probe, protein transduction domain-oxygen-dependent degradation domain-HaloTag-Rhodamine (POH-Rhodamine). In this study, we observed the localization of HIF-1α proteins by immunohistochemistry and the fluo...

With the rapid progress of tissue engineering and regenerative medicine, the cell-friendly construction of a 3D extracellular matrix in a precisely controlled manner is needed. This biomimicry of the native extracellular matrix replicates major aspects of the native cellular microenvironment. Herein, we design a polyethylene glycol (PEG) based hydrogel combined with caging chemistry, which coul...

The formation of high-density silver nanoparticles and a novel method to precisely control the spacing between nanoparticles by temperature are demonstrated for a tunable surface enhanced Raman scattering substrates. The high-density nanoparticle thin film is accomplished by self-assembling through the Langmuir-Blodgett (LB) technique on a water surface and transferring the particle monolayer t...

Previously we demonstrated that calmodulin binds to the carboxy terminus of Spc110p, an essential component of the Saccharomyces cerevisiae spindle pole body (SPB), and that this interaction is required for chromosome segregation. Immunoelectron microscopy presented here shows that calmodulin and thus the carboxy terminus of Spc110p localize to the central plaque. We created temperature-sensiti...

Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and...

A first-principles pseudopotential study of neutral self-interstitial defects in silicon is reported, together with calculations for Pandey’s concerted exchange mechanism for selfdiffusion. The energies and structures of the fully relaxed hexagonal, tetrahedral, split-〈110〉, ‘caged’ (Clark S J and Ackland G J 1997 Phys. Rev. B 56 47), split-〈100〉, and bond-centred interstitials are calculated u...

The existence of several periodic structures (known as gliders) in the evolution space of the one-dimensional cellular automaton Rule 110, has important lines of investigation in cellular automata theory such as: complex behavior, universal computation and self-reproduction. We investigate the types of gliders, their properties and production through collisions in Rule 110 and their representat...