These analyses show that this cyclic A M P phosphodiesterase.contains at least 15ng of zinc per activity unit. The sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of fraction 18 suggests that the specific activity of pure enzyme is at least 104 units/mg, and the results of Fujimoto et al . (1974), obtained at 37"C, suggest it may be as high as 160 units/mg at 30°C. Thus 1 mol (65000g...