In hyperspectral image unmixing, a collection of pure spectra, the so-called endmembers, is identified and their abundance fractions are estimated at each pixel. While endmembers are often extracted using a geometric approach, the abundances are usually estimated using a least-squares approach by solving an inverse problem. In this paper, we tackle the problem of abundance estimation by using a...

The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFPs) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaff...