objective: this study presents an efficient, cost-effective method to improve proliferation and colonization of spermatogonial stem cells (sscs) in vitro. methods: isolated sscs from neonate mice were cultured in dmem culture medium with 10% fetal bovine serum (fbs). in the first phase of the study, the temperature was controlled by low intensity pulsed ultrasound stimulation (lipus) of the pla...

In the complicated process of bone regeneration, osteogenic differentiation and angiogenesis are crucial. this study, a novel dual small molecule composite electrospun scaffold was prepared using polycaprolactone embedded with angiogenic factor tauro-ursodeoxycholic acid dexamethasone to promote early osteogenesis. Additionally, related properties, activity, activity analyses were conducted for...

Within the DFR concept, the methodology of Design of Experiments (DOE) can be applied in the design and manufacturing stages to improve product reliability. It can be used to systematically investigate the variables (factors) that influence the life of the product, thereby providing the analyst with information that can be used to improve reliability. The analysis can also be used in the produc...

in this research, the accelerated ripening of the kabkab dates from khalal to tamr using nacl and acetic acid solutions was studied. fruits at khalal stage were harvested and washed with distilled water before being treated with ripening inducers: 1. nacl solutions, 2. acetic acid solutions and 3. aqueous solution of nacl (1%) and acetic acid. moisture, ph, colour, brix and textural firmness of...

Putrescine (diaminobutane) was previously found to stimulate proliferation of human fibroblasts in tissue culture, and a growth factor produced by these cells was identified as putrescine. In the present paper putrescine transport is studied. The rate of putrescine transport was dependent on temperature, and most of the labeled putrescine was retained by the cells after washing with excess unla...

Semi-automatic analysis of the repair process of a circular mechanical lesion of confluent human vascular endothelial cells in vitro was used to evaluate the contributions of cell migration and cell proliferation. Standard heparin added to culture medium that contained 30% human serum induced an inhibition of cell migration at the lesion margin during the first day after injury. Several sulfate...

abstractbackground: fetal bovine serum (fbs) is widely used in cell culture laboratories,risk of zoonotic infections and allergic side effects create obstacles for its use inclinical trials. therefore, an alternative supplement with proper inherent growthpromotingactivities is demanded.objective: to find fbs substitute, we tested human umbilical cord blood serum(hucs) for proliferation of human...

Infants infected with HIV have a more severe course of disease and persistently higher viral loads than HIV-infected adults. However, the underlying pathogenesis of this exacerbation remains obscure. Here we compared the rate of CD4(+) and CD8(+) T-cell proliferation in intestinal and systemic lymphoid tissues of neonatal and adult rhesus macaques, and of normal and age-matched simian immunodef...