The numbers of alpha-naphthyl acetate esterase (ANAE)-containing cells (other than T lymphocytes) in non-Hodgkin's lymphomas (NHL) and reactive lymph nodes have been counted, using the Reichert-Jung (Kontron) MOP-AMO3 user-controlled image analyzer. Twenty specimens of NHL and ten reactive nodes were examined. Cells were demonstrated by their content of acid alpha-naphthyl acetate esterase (ANA...

The four most important non-specific carboxylesterases from rat liver were assayed for their ability to hydrolyse retinyl esters. Only the esterases with pI 6.2 and 6.4 (= esterase ES-4) are able to hydrolyse retinyl palmitate. Their specific activities strongly depend on the emulsifier used (maximum rate: 440 nmol of retinol liberated/h per mg of esterase). Beside retinyl palmitate, these este...

The presence of organophosphate pesticides could inhibit the activity alpha-naphthyl acetate esterase (ANAE), which use as fundamental for biosensor applications. k-carrageenan was used immobilization ANAE extracted from wheat-based sources. free and immobilized assessed optimum at a range temperature pH. Enzyme kinetic studies were performed using Michaelis-Menten parameters Lineweaver-Burk, L...

tetranychus urticae koch is an important and destructive pest of crops, fruits and ornamental plants in worldwide. the high fecundity of t. urticae and its abruptly life span as well as the extensive application of acaricides for keeping t. urticae damages below economic injury level, caused to resistance to acaricides and control failures. in this research the fumigant activity of essential oi...

Germinating seeds of Tamarindus indica synthesizes various enzymes which are required for the degradation of seed reserves such as xyloglucans, fatty acid esters and proteins. Among these, esterases, belonging to a group of hydrolytic enzymes catalyze the hydrolysis of various types of esters. They play an important role in cell expansion as well as detoxification of xenobiotics and many agroch...

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

Lymphocytes from a case of B-cell chronic lymphocytic leukemia (CLL) were obtained in a highly purified state from a therapeutic leukapheresis preparation. The CLL lymphocytes showed a fine, scattered, granular pattern of nonspecific esterase cytochemical reactivity with either alpha-naphthyl acetate (alpha NA) or alpha-naphthyl butyrate (alpha NB) substrate as opposed to the more focal pattern...

Esterases of 85strains of the four biochemically-defined subgenera of Salmonella, when analysed by the acrylamide-agarose zymogram technique using several synthetic substrates, gave four principal bands (E1, E2, E3, E4) and two minor ones. The E1 esterase band hydrolysed alphs-naphthyl acetate, whereas the E2 band hydrolysed beta-naphthyl acetate. These bands were resistant to di-isofluoropropy...

Naked neck fowl (Gallus gallus domesticus) comprises one of the indigenous chicken populations in Bangladesh. The present investigation was conducted to study the polymorphic pattern of the estrase isozyme in different tissues of the fowl by polyacrylamide gel electrophoresis (PAGE) system. In this experiment, α-napthyle and β-napthyle acetate were used as substrate. Esterase variability of dif...