1. The incorporation of d-[1-(14)C]mannose, d-[2-(3)H]mannose and N-acetyl-d-[1-(14)C]-glucosamine into glycoproteins and lipid-linked intermediates of mammary explants obtained from lactating rabbits was studied. The amount of radioactivity incorporated into lipid-linked intermediates was very low compared with the incorporation into protein. Most of the radioactivity incorporated into the chl...

Aerobic and anaerobic bacteria isolated from human axillae were tested for their capacity to adhere to buccal epithelial cells, immortalized human epithelial (HEp-2) cells, and undifferentiated and differentiated human epithelial cells. In general, both aerobic and anaerobic diphtheroids adhered better to differentiated human epithelial cells than to HEp-2 and undifferentiated human epithelial ...

l-Glutamine d-fructose 6-phosphate amidotransferase (EC 2.6.1.16) was extracted and purified 600-fold by acetone fractionation and diethylaminoethyl cellulose column chromatography from mung bean seeds (Phaseolus aureus). The partially purified enzyme was highly specific for l-glutamine as an amide nitrogen donor, and l-asparagine could not replace it. The enzyme showed a pH optimum in the rang...

During first gamete interaction, oligosaccharides on the glycoproteins present in oocyte envelopes of the rock shrimp, Rhynchocinetes typus, play an important role in spermatozoon recognition prior to the onset of penetration. These oligosaccharides have terminal monosaccharides that may be recognized by means of specific lectins. A variety of marine species have monosaccharides on their oocyte...

the first enzyme of the pathway for uridine diphosphate n-acetyl-d-glucosamine (udpag) biosynthesis i.e. l-glutamine: d-fructose 6-p amidotransferase (e.c. 2.6.1.16) was purified 52-fold from human placenta using methanol fractionation and column chromatography on deae-sephadex a-50. the enzyme showed optimal activity in a broad range of ph from 5.8 to 7.8 in both phosphate and cacodylate buffe...

Herbaspirillum seropedicae is a plant growth-promoting diazotrophic betaproteobacterium which associates with important crops, such as maize, wheat, rice and sugar-cane. We have previously reported that intact lipopolysaccharide (LPS) is required for H. seropedicae attachment and endophytic colonization of maize roots. In this study, we present evidence that the LPS biosynthesis gene waaL (code...

Multiple fluorination of glycostructures has emerged as an attractive way modulating their protein affinity, metabolic stability, and lipophilicity. Here we described the synthesis a series mono-, di- trifluorinated N -acetyl-?-glucosamine ?-galactosamine analogs. The key intermediates are corresponding multiply fluorinated glucosazide galactosazide thioglycosides prepared from deoxyfluorinated...

With N-acetyl-D-glucosamine 8....................................................................... S31 With D-glucose 9................................................................................................ S33 With methyl-β-D-glucoside 10......................................................................... S35 With L-mannose 12.......................................................

A systematically varied series of conformationally restricted ketones, readily prepared from N-acetyl-D-glucosamine, were tested against representative olefins as asymmetric epoxidation catalysts showing useful selectivities against terminal olefins and, in particular, typically difficult 2,2-disubstituted terminal olefins.

Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and ...