The molecular control of the distribution and motion of acetylcholine receptors in the plasma membrane of developing rat myotubes in primary cell culture was investigated by fluorescence techniques. Acetylcholine receptors were marked with tetramethylrhodamine-labeled a-bungarotoxin and lateral molecular motion in the membrane was measured by the fluorescence photobleaching recovery technique. ...

This study was aimed at evaluating the effect of Dihydroartemisinin (DHA)on contractility to drugs in ileum isolated smooth muscle preparations guinea pigs using Vitro experimental procedures / standard protocols an organ bath set up. dihydroartemisinin (1.0 x 10 - 8 1.0 – 5mg/mL) when applied alone and separately excited marked variable effects on pig . In some preparation it showed no respons...

The action of acetylcholine (10(-9)-10(-4) M) was investigated in isolated rabbit cardiac Purkinje fibers, using standard microelectrode recording of transmembrane potentials and two-microelectrode voltage clamp technique. In nonstimulated fibers, acetylcholine hyperpolarized the diastolic membrane potential and slowed or suppressed spontaneous activity. The hyperpolarization was more pronounce...

Effects of venom from black scorpion Androctonus crasicuda (AC) were determined on isolated chick biventer cervices nerve-muscle and mouse hemidiaphragm preparations using twitch tension method. The isolated nerves were stimulated by electrical stimulator and response to each stimulus was recorded. The venom mainly acted prejunctionally to facilitate neuromuscular activity due to an increase in...

Nerve and muscle cells from clonal lines interact in vitro, resulting in the association on the muscle surface of an area of increased acetylcholine sensitivity with a site of nerve-muscle contact. This localization of acetylcholine sensitivity on the muscle cell to a site of contact between nerve and muscle was found to occur when acetylcholine receptors on the muscle had been blocked with alp...

Acetylcholine was identified and quantified by g.l.c. in moss callus. The acetylcholine concentrations in the moss callus were found to be regulated by phytochrome. The highest concentrations of acetylcholine occurred after irradiation with red light. Moss callus grown in the dark contained no acetylcholine.

INTRODUCTION It has been known for several years that epinephrine is stored with ATP and there have been several NMR studies of this complex (1,2). Until recently the nature of acetylcholine storage vesicles has remained a mystery. It has now been demonstrated that acetylcholine, in some systems, is stored with a specific protein and ATP (3,4). While a previous study failed to show any interact...

BACKGROUND AND PURPOSE We tested the hypothesis that activation of phosphatidylinositol (PI) 3-kinase is involved in dilator responses of the basilar artery to acetylcholine in vivo. METHODS Responses of the basilar artery were measured by the cranial window technique in anesthetized rats. To examine the role of PI 3-kinase in acetylcholine-induced calcium signaling, we measured intracellular...

This study examined the distribution of muscarinic acetylcholine receptor-immunoreactive neurons in the amygdaloid complex of the rat, with emphasis on the central nucleus. The monoclonal antibody M35 raised against purified muscarinic acetylcholine receptor protein was used to visualize muscarinic acetylcholine receptor-immunoreactive cells. Muscarinic acetylcholine receptor immuno-reactivity ...

Acetylcholine is the neurotransmitter of parasympathetic nervous system, synthesized from choline and involved in several neurodegenerative diseases. Exploration cholinergic neurotransmission human central system limited by lack a sensitive specific method for determination acetylcholine expression. We developed an hydrophilic interaction liquid chromatography – mass spectrometry quantification...