In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...

AIMS To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS Best results were obtained by auramine O staining afte...

Background: Lymph node tuberculosis (LNTB) is the leading form of extra pulmonary tuberculosis and common in our country. Acid Fast Bacilli (AFB) detection by Ziehl-Neelsen (ZN) method in the fine needle aspirate from these lymph nodes is confirmatory. Objective: To assess the diagnostic utility of Ziehl-Neelsen staining in lymphadenopathy cases, since it is commonly used method in the diagnosi...

the aim of this study was to analyse polar lipids of candida species isolated from ahwaz (iran) by fast atom bombardment mass spectrometry (fab ms). nine isolates of candida sp. were identified by growth at 45°c, production of chlamydoconidia on cornmeal agar, colonial colour on chromagar candida, germ tube production and id 32c kits. then polar lipids were extracted from freeze-dried cultures ...

A retrospective study of various diagnostic postmortem techniques used in a 4-year surveillance program for detection of Mycobacterium bovis infection in wild white-tailed deer (Odocoileus virginianus) was conducted. The tests evaluated were routine histopathology, acid-fast staining, detection of acid-fast bacilli in culture, and an M. tuberculosis group-specific genetic probe applied to pure ...

Background and objectives: Cancer patients treated with chemotherapy and other immunosuppressive drugs are always prone to various infections including opportunistic parasites. Since detection of infections in immunocompromised patients are frequently imperfect and the usual symptoms such as pyrexia are missing or hidden due to leukopenia, the importance of detection of opportu...

As infection with Cryptosporidium parvum becomes increasingly recognized as a cause of outbreaks of foal diarrhea, a rapid method of oocyst detection in equine feces that can be done easily in the practice setting is desirable. Protozoal identification techniques that have been described include the evaluation of stained fecal smears or preparations of floated feces, using both light and phase-...