Lysosomal acid phosphatase levels are reduced in murine macrophages by virulent strains of Nocardia asteroides. At the same time, other lysosomal enzymes either remain unchanged or increase in activity, indicating that acid phosphatase is not lost because of degranulation or membrane leakage. This study shows that acid phosphatase was utilized as a sole carbon source by Nocardia asteroides and ...

”he acid phosphatase activity that is elevated 3to 5fold in the spleen in Gaucher’s disease can be resolved into two principal isoenzymes by chromatography on sulphopropyl-Sephadex: one tartrate-sensitive component does not bind to the resin whereas the second acid phosphatase, which is tartrate-resistant, binds to the column and can be eluted by a buffer containing 0.4 M sodium chloride. We ha...

In this study, we isolated cDNA encoding lysophosphatidic acid (LPA) phosphatase (LPAP). The amino acid sequence deduced from the cDNA encoding LPAP had 421 residues including a putative signal peptide and was homologous to acid phosphatase, especially at the active site. Human LPAP had 28.5% amino acid identity to human prostatic acid phosphatase. Northern blot analysis showed a ubiquitous exp...

It is shown here that treatment of metaphase-arrested HeLa cells with okadaic acid (0.15-2.5 microM) leads to dephosphorylation of histone H1. This effect is presumably due to the specific ability of okadaic acid to inhibit protein phosphatases 1 and/or 2A, because okadaic acid tetraacetate, which is not a phosphatase inhibitor, has no effect. Dephosphorylation of H1 does not occur if okadaic a...

Background: There are multiple diagnostic tests and procedures are available for diagnosis of infertility but semen analysis still is considered as most valuable diagnostic test for investigation of infertility.it is equally important that the semen analysis should be performed by experienced and expert person by proper technique. Biochemical analysis seminal fluid provide valuable information ...

-Plant roots and soil organisms increase phosphorus availability by releasing acid and alkaline phosphatase. Organic phosphorus compounds are hydrolysed into plant-available forms through the action of these enzymes. Phosphatase is of particular importance under conditions of low phosphorus availability which is commonly found in pinon-juniper soils. Acid and alkaline phosphatase activities (mg...

Alterations for acid and alkaline phosphatase levels and their pattern of splenic and lymph node activity in normal and virus-induced lymphoblastic leukemia were studied. Enzyme levels were examined by using both cytochemical and biochemical procedures. The GC leukemia virus, a ribonucleic acid murine virus antigenically related to the Rauscher-Moloney viruses, was used to stimulate acid and al...

Virulent and avirulent clones of Leishmania donovani promastigotes were examined for their acid phosphatase activity. The acid phosphatase activity of whole-cell lysates of virulent clones was 1.5 to 2.0 times higher than that of avirulent clones. Pellet fractions (260,000 x g, 30 min) from sonicated promastigotes of a virulent clone and an avirulent clone contained 60 and 40% of the total enzy...

Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, its dysfunction may lead to series diseases. A novel dual-signal fluorescence method for acid detection based on europium polymer (europium-pyridine dicarboxylicacid-adenine) pyridoxal phosphate (PLP) was proposed. PLP coordinated with via Eu3+ P–O bonds, the quenched due photoinduced electron transfer (PET...