pH is an important control parameter for maintenance of cell viability and tissue functions. pH monitoring provides valuable information on cell metabolic processes and the living environment. In this study, we prepared dual pH-sensitive, fluorescent dye-loaded polypeptide nanoparticles (DPNs) for ratiometric sensing of pH changes in living cells. DPNs contain two types of dyes: N-(rhodamine B)...

INTRODUCTION The aim of this study was to evaluate the compressive strength of calcium-enriched mixture (CEM) cement in contact with acidic, neutral and alkaline pH values. METHODS AND MATERIALS The cement was mixed according to the manufacturer's instructions, it was then condensed into fourteen split molds with five 4×6 mm holes. The specimens were randomly divided into 7 groups (n=10) and ...

A 1.1 × 106 Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC33960 with a production of 30 mg L-1 when grown in AS-168 medium, which mainly composed of mannose and galactose with a small amount of glucose in a molar ratio of 55.9 : 43.2 : 0.9. Two glycosyltransferase genes (HAH_1662 and HAH_1667) were identified to be responsible for ...

objective: the purpose of this study was to evaluate the solubility of three luting cements in artificial saliva. materials and methods: twenty disks (10×4 mm) of polycarboxylate, zinc phosphate and glass ionomer cements were prepared according to manufactures’ instructions. after setting, they were desiccated and each ten specimens were immersed in artificial saliva with special ph (3 or 5), c...

iron is essential to erythrocyte oxygen transport and is a catalyst for oxidative metabolism in all cells. iron is absorbed by the duodenum and requires an acidic environment for optimal absorption. iron is found for 70% in erythrocytes, and 30% in storage, and a small amount in myoglobin and cytochromes. iron supplementation reduces anemia in breastfed infants and increases in significant dose...

  Objective: Orthodontic wires should have high resistance against corrosion in the oral environment. Since the effect of pH on corrosion has been well recognized, this study sought to assess and compare the electrochemical corrosion of orthodontic brackets and wires of different brands in acidic artificial saliva.   Methods: This in vitro experimental study was conducted on 24 mandibular centr...

Flavodoxin is an essential protein for Helicobacter pylori, a pathogen living in the very acidic environment of the gastric tract and responsible for several diseases. We report the conformational stability of the protein in neutral and acidic pH. The apoprotein remains native between pH 12 and 5 and adopts a monomeric molten globule conformation at more acidic pH values. The equilibrium unfold...