DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-...

this study deals with bioleaching of the low grade uranium ore of saghand mine in iran using a mixed culture of mesophilic bacteria (acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and leptospirillum ferrooxidans). experiments were carried out in shaking flask examining the effect of temperature, initial ferrous ion concentration, pulp density and particle size. variations of ph, ...

Studies of the obligate chemoautotrophic iron bacteria have been largely descriptive in character. This situation is not due to any lack of interest in the physiology and biochemistry of these bacteria, but rather to the inability to grow them in large numbers, and the attendant problem of harvesting cells free from the voluminous precipitate of iron that accompanies growth. Silverman and Lundg...

the effects of different factors on growth and bio-oxidation efficiency of a native strain of acidithiobacillus ferrooxidans have been evaluated by the utilization of response surface methodology, rsm. medium ph and iron concentration were found to be the most significant factors while temperature and ammonia concentrations had the least weight within the ranges investigated. optimum operationa...

Acidithiobacillus ferrooxidans is an extremophilic bacterium used in biomining processes to recover metals. The presence in A. ferrooxidans ATCC 23270 of canonical copper resistance determinants does not entirely explain the extremely high copper concentrations this microorganism is able to stand, suggesting the existence of other efficient copper resistance mechanisms. New possible copper resi...

The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans is of great importance in biomining operations. During the bioleaching of ores, microorganisms are subjected to a variety of environmental stresses and to the limitations of some nutrients, such as inorganic phosphate (P(i)), which is an essential component for all living cells. Although the primary source of phosphorus for micr...

Extremely acidophilic microorganisms (pH optima for growth of ≤3) are utilized for the extraction of metals from sulfide minerals in the industrial biotechnology of "biomining." A long term goal for biomining has been development of microbial consortia able to withstand increased chloride concentrations for use in regions where freshwater is scarce. However, when challenged by elevated salt, ac...

The variety of kinetics expressions encountered in the literature and the unreasonably broad range of values reported for the kinetics constants of Acidithiobacillus ferrooxidans underscore the need for a unifying experimental procedure and for the development of a reliable kinetics equation. Following an extensive and critical review of reported experimental techniques, a method based on batch...

By proteomic analysis we found a 21-kDa protein (P21) from Acidithiobacillus ferrooxidans ATCC 19859 whose synthesis was greatly increased by growth of the bacteria in pyrite, thiosulfate, elemental sulfur, CuS, and ZnS and was almost completely repressed by growth in ferrous iron. After we determined the N-terminal amino acid sequence of P21, we used the available preliminary genomic sequence ...