Background: Laboratory diagnosis of malaria is currently recommended for the confirmation of the disease before management. The two most common techniques in use for the diagnosis of malaria parasite in Sudan are Giemsa stained smears and Rapid diagnostic tests(RDTs). This study was carried out to compare microscopy ( Giemsa and Acridine orange stain ) and RDTs as effective tools for the diagno...

It has long been known that certain organic dyes can photosensitize cells, leading to cellular damage or death. Recently, studies were performed on one such dye, Toluidine Blue, to determine the mechanism of its lethal photosensitization of bacteria (M. M. Mathews, J. Bacteriol. 85:322, 1963). These studies showed that the cellular site of the lethal action was the cytoplasmic membrane and its ...

LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...

LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...

The in vitro localization of acridine orange (AO) in living cells was monitored by means of fluorescence microscopy, quantitative cell viability studies, and photofluorimetric measurements following dye-cell interaction. The parameters, pH, time, dye concentration, and the metabolic state of the cell were found to exert a profound influence on the time course and distribution of staining. The p...

The triplet state of acridine orange dissolved in methanol/water matrix was investigated by ESR. In absence of oxygen a strong temperature dependence of the spectra was observed. At low temper­ ature (100 °K) the zero-field splitting parameters calculated from the triplet spectrum are: X /h c = 0.0050 cm-1 , Y / h c = 0.0342 cm-1 , Z //ic = 0.0387 cm-1 , at higher temperature (140 °K) : X * ( h...

Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...