Abstract Separation of seed mixtures for the purpose their purification or calibration by fractions is a mandatory technological operation in processing most crops. Due to its properties, process separation individual crops has undefined parameters low efficiency flow. A similar material safflower, which were determined work taking into account different methods components mixture. The involves...

Adenosine-to-inosine RNA editing modifies maturing mRNAs through the binding of adenosine deaminase acting on RNA (Adar) proteins to double-stranded RNA structures in a process critical for neuronal function. Editing levels at individual editing sites span a broad range and are mediated by both cis-acting elements (surrounding RNA sequence and secondary structure) and trans-acting factors. Here...

Several prothrombin activators from snake venoms have been isolated and characterized (For details, see 1). Initially, they were classified into three major groups based on their structural and functional properties (1, 2). They differ from each other in their cofactor requirements and the products formed. Group I activators are metalloproteinases. They convert prothrombin to meizothrombin and ...

Stereoregulated diribonucleoside phosphorothioates were synthesized by the use of 2'-O-TBDMS-protected ribonucleoside 3'-O-oxazaphospholidine derivatives as monomers and N-(cyanomethyl)ammonium salts as activators. Diastereoselectivity of the condensation reaction was found to be highly dependent on the substituent groups of the oxazaphospnolidine ring as well as the structure of the activators...

Metal-based bifunctional methane activators are modeled utilizing density functional theory. The impact upon activation barriers of the metal among late 3d MII ions (Co – Zn) is quite pronounced, ca. 7 kcal/mol. Alteration metal’s coordination environment without changing supporting ligand types (e.g., comparing cis and trans isomers) has a computed on ± 1 One significant result involves varyin...

This work studies the feasibility to employ a combination of volcanic ash (natural waste) with different raw materials in production geopolymers: fly and mining tailing (considered hazardous solid waste), natural pozzolan, metakaolin. study compares properties geopolymers based on ash, metakaolin, relation 1:1 addition NaOH 15M Na2SiO3 as alkali activators. FTIR XRD assays mechanical tests were...

Alkali-activated binders have been considered a low carbon alternative to cement and are produced by reacting aluminosilicate precursor with an alkali-activator. However, alkali-activators observed be major contributor the environmental burdens of alkali-activated materials regarding various impact categories. Therefore, this study aims perform assessment using life cycle methodology on mortars...

BACKGROUND Transcription by RNA polymerase II in eukaryotic cells requires the ordered assembly of general transcription factors on the promoter to form a preinitiation complex. Transcriptional activator proteins (activators) stimulate transcription by increasing the rate and/or extent of preinitiation complex assembly. We have shown previously that acidic activators increase the stable associa...

DNA methylation and chromatin modification operate along a common pathway to repress transcription; accordingly, several experiments demonstrate that the effects of DNA methylation can spread in cis and do not require promoter modification. In order to investigate the molecular details of the inhibitory effect of methylation, we microinjected into Xenopus oocytes a series of constructs containi...

Cell-based splicing of minigenes is used extensively in the analysis of alternative splicing events. In particular, such assays are critical for identifying or confirming the in vivo relevance of cis- and trans-acting factors in the regulation of particular splicing patterns. Here we provide detailed information on the methods specific to the cell-based analysis of minigene splicing. In additio...