Phosphorylation/dephosphorylation events in human blood platelets were investigated during their adhesion to collagen under flow conditions. Using 32P-labeled platelets and one-dimensional gel electrophoresis, we found that adhesion to collagen mediated primarily by the alpha2beta1 integrin resulted in a strong dephosphorylation of several protein bands. Neither adhesion to polylysine nor throm...

The urokinase-type plasminogen activator receptor (uPAR) is a glycosylphosphatidyl inositol-anchored protein that mediates cell adhesion to the extracellular matrix protein vitronectin (VN). We demonstrate here that this cell adhesion process is accompanied by the formation of an adhesion patch characterized by an accumulation of uPAR into areas of direct contact between the cell and the matrix...

The adhesion of hemocytes plays an important role in the innate immunity of abalones. According to the results from this study, several conclusions about the role of PKA (protein kinase A) in the adhesion of abalone hemocytes. First, during cell adhesion, the amount of cAMP decreased in hemocytes. Second, elevation of cAMP inhibited cell adhesion. Third, the PKA inhibitor KT5720 enhanced the ce...

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

in eukaryotes, vesicle trafficking is regulated by the small monomeric gtpases of the rab protein family. rab11, (a subfamily of the ypt/rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric rab gtpases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. in an earlier communication, we have shown th...

anti-adhesion therapy represents a potentially promising avenue for the treatment and prevention of tuberculosis in a post-antibiotic era. adhesins are surface-exposed microbial structures or molecules that enable pathogenic organisms to adhere to host surfaces, a fundamental step towards host infection. although several mycobacterium tuberculosis adhesins have been identified, it is predicted ...

Abstract Pancreatic ductal adenocarcinoma (PDAC) is the most common form of pancreatic cancer with an abysmal prognosis rate over last few decades. Early diagnosis and prevention could effectively combat this malignancy. Therefore, it crucial to discover potential biomarkers identify asymptomatic premalignant or early malignant tumors PDAC. Gene expression analysis a powerful technique candidat...