PURPOSE Adoptive transfer of autologous tumor infiltrating lymphocytes (TIL) can mediate durable cancer regression in selected patients with metastatic melanoma. However, the tumor antigens associated with these favorable responses remain unclear. We hypothesized that a clinical strategy involving the iterative adoptive transfer of selected autologous antigen-specific T-cell clones could help s...

Systemic delivery of lentiviral vector (LV) in immunocompetent mice leads to efficient in vivo cell transduction and expression of the encoded protein under the control of the ubiquitous promoter of human cytomegalovirus (CMV). However, antitransgene immune response results in clearance of transduced cells 4 weeks after injection. T regulatory cells (Tregs), which have been demonstrated to cont...

Stress catabolism and systemic inflammatory response is ubiquitous in critically ill patients. Multiorgan failure, various morbidities, higher length of stay are commonly seen the ill, especially current pandemic context. Nutritional importance such critical-care settings immense. Adequate nutrition care helps overcome stress modulates both innate adoptive immunity favorably, loss body muscle m...

Control of well drilling operations poses a challenging issue to be tackled. The loss of well control could lead to the occurrence of blowout as a severe threat, involving the risk of human lives and environmental and economic consequences. Conventional proportional-integral (PI) controller is a common practice in the well control operation. The small existing margin between pore pressure and f...

Tumor necrosis factor alpha (TNFα) and its two receptors (TNFR1 and 2) are known to stimulate dendritic cell (DC) maturation and T cell response. However, the specific receptor and mechanisms involved in vivo are still controversial. Here we show that in response to an attenuated mouse hepatitis virus (MHV) infection, DCs fail to mobilize and up-regulate CD40, CD80, CD86, and MHC class I in TNF...

TNF-α and its two receptors (TNFR1 and 2) are known to stimulate dendritic cell (DC) maturation and T cell response. However, the specific receptor and mechanisms involved in vivo are still controversial. In this study, we show that in response to an attenuated mouse hepatitis virus infection, DCs fail to mobilize and up-regulate CD40, CD80, CD86, and MHC class I in TNFR1(-/-) mice as compared ...

The adoptive transfer of antigen-specific effector T cells is being used to treat human infections and malignancy. T cell persistence is a prerequisite for therapeutic efficacy, but reliably establishing a high-level and durable T cell response by transferring cultured CD8(+) T cells remains challenging. Thus, strategies that promote a transferred high-level T cell response may improve the effi...

Thymus-dependent, helper cells ("T" lymphocytes) 1 and thymus-independent, antibody-forming precursor cells ("B" lymphocytes) cooperate in the initiation of the primary antibody response of rodents to a variety of antigens (1-3). Both cell lines also cooperate in the initiation of the secondary antibody response, and both carry immunological memory (4, 5). Recent cell transfer studies indicate ...

Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (N...