نتایج جستجو برای: affinity ligands

تعداد نتایج: 180734  

Journal: :Bioinformation 2008
Alisa Wilantho Sissades Tongsima Ekachai Jenwitheesuk

Virtual drug screening using protein-ligand docking techniques is a time-consuming process, which requires high computational power for binding affinity calculation. There are millions of chemical compounds available for docking. Eliminating compounds that are unlikely to exhibit high binding affinity from the screening set should speed-up the virtual drug screening procedure. We performed dock...

Journal: :Molecular cancer therapeutics 2016
Yangmi Lim Jiho Yoo Min-Soo Kim Minkyu Hur Eun Hee Lee Hyung-Suk Hur Jae-Chul Lee Shi-Nai Lee Tae Wook Park Kyuhyun Lee Ki Hwan Chang Kuglae Kim YingJin Kang Kwang-Won Hong Se-Ho Kim Yeon-Gil Kim Yeup Yoon Do-Hyun Nam Heekyoung Yang Dong Geon Kim Hyun-Soo Cho Jonghwa Won

The EGFR-targeted monoclonal antibodies are a valid therapeutic strategy for patients with metastatic colorectal cancer (mCRC). However, only a small subset of mCRC patients has therapeutic benefits and there are high demands for EGFR therapeutics with a broader patient pool and more potent efficacy. In this study, we report GC1118 exhibiting a different character in terms of binding epitope, a...

2015
Agata Levay Randall Brenneman Jan Hoinka David Sant Marco Cardone Giorgio Trinchieri Teresa M. Przytycka Alexey Berezhnoy

Oligonucleotide aptamers represent a novel platform for creating ligands with desired specificity, and they offer many potentially significant advantages over monoclonal antibodies in terms of feasibility, cost, and clinical applicability. However, the isolation of high-affinity aptamer ligands from random oligonucleotide pools has been challenging. Although high-throughput sequencing (HTS) pro...

Journal: :Protein expression and purification 2014
Ward G Walkup Mary B Kennedy

PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ∼90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholipids. We hypothesized that the specific, strong interactions of PDZ domains with their ligands woul...

2014
Vinod Kumar Irna E. Ridzwan Konstantinos Grivas John W. Lewis Mary J. Clark Claire Meurice Corina Jimenez-Gomez Irina Pogozheva Henry Mosberg John R. Traynor Stephen M. Husbands

Emerging clinical and preclinical evidence suggests that a compound displaying high affinity for μ, κ, and δ opioid (MOP, KOP, and DOP) receptors and antagonist activity at each, coupled with moderate affinity and efficacy at nociceptin opioid peptide (NOP) receptors will have utility as a relapse prevention agent for multiple types of drug abuse. Members of the orvinol family of opioid ligands...

Journal: :International Journal of Peptide Research and Therapeutics 2022

Abstract Recently, de novo MS/MS peptide sequencing has enabled the application of affinity selections to synthetic mixtures that approach diversity phage libraries (> 10 8 random peptides). In conjunction with ‘split-mix’ solid phase synthesis access equimolar mixtures, this provides a straightforward means examine considerably higher than been feasible historically. Here, we offer critical...

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