Aflatoxins, a group of structurally related mycotoxins, are well known for their toxic and carcinogenic effects in humans and animals. Aflatoxin derivatives and protein conjugates are needed for diverse analytical applications. This work describes a reliable and fast synthesis of novel aflatoxin derivatives, purification by preparative HPLC and characterisation by ESI-MS and one- and two-dimens...

Rat cecal microflora from high- and low-fiber-fed animals hydrolyzed aflatoxin conjugates to metabolites indistinguishable from aflatoxin B1 and aflatoxin P1, but aflatoxicol was not a transformation product.

2-Mercaptoethanol inhibits growth of Aspergillus parasiticus NRRL 3240 and aflatoxin formation by the fungus. When added to the resuspended medium, 2-mercaptoethanol inhibited [1-14C]acetate incorporation into both aflatoxins and neutral lipids, thereby showing that it acts at an early stage of aflatoxin biosynthesis. The inhibition is probably due to its chelating action on zinc, which is esse...

Efficacy of some local plants in the management of Aspergillus flavus and aflatoxin contamination was investigated. Aqueous extracts of rhizome of Zingiber officinalis and leaf extracts of Trigonella foenum-graecum inhibited aflatoxin production to a significant level. While solvent extracts of Zingiber officinalis and Oxalis corniculata could suppress both the growth and aflatoxin production b...

A method has been developed for the production of aflatoxin by growing Aspergillus flavus NRRL 3145 on solid substrate wheat. Optimal yields of 900 mug of aflatoxin G(1) and 900 mug of aflatoxin B(1) per g of substrate were obtained in 4 to 5 days at 28 C. A study of aflatoxin production on hulls and groats of oats and on whole oats by A. flavus strains NRRL 2999, NRRL 3000, and NRRL 3145 revea...

in this investigation aflatoxin contamination of eighty different iranian white cheeses were determined by t.l.c. method. the result of this study showed that, 97.5% of the samples contained aflatoxin m1 & m2 which are transferred from milk. as far as direct contamination, 30% of the samples contained aflatoxin b1, g1, g2, from which 2.5% contained aflatoxin b1, 5% contained aflatoxin b2, 20% c...

The interactions of aflatoxin B(1) with certain histone fractions and DNA were investigated by means of viscosity measurements and equilibrium dialysis. Two main histone fractions (F2b and F1), both lysine-rich, were examined after treatment with the toxin. Fraction 2b and 1 differ in amino acid composition and behave differently, in regard to gross conformation, in the presence of electrolytes...

Aflatoxins are toxic and carcinogenic polyketide metabolites produced by certain fungal species, including Aspergillus flavus and A. parasiticus. Many internal and external factors, such as nutrition and environment affect aflatoxin biosynthesis; therefore, we analyzed the transcriptome of A. flavus using expressed sequence tags (ESTs) from a normalized cDNA expression library constructed from ...

Several phenols and related compounds have been shown to have detrimental effects on insects while others have antibiotic activity against fungi which attack higher plants. Insects have also been implicated as contributors to preharvest contamination of corn [maize], Zea mays L, by aflatoxin. The objectives, therefore, were to determine (a) if commercial corn hybrids vary in their silk maysin c...

Suimmtl7iarv. The interactions of aflatoxin B, with certain histone fractions and DNA were investigated by means of viscosity measurements and equiilibriuim dialysis. Two main histone fraction,s (F2b and Fl), both lysine-rich, were examined after treatment with the toxin. Fraction 2b and 1 differ in amino acid composition an(l behave differently, in regard to gross conformation, in the presence...