PURPOSE Signal transducer and activator of transcription protein-3 (STAT3) is a transcription factor that participates in many biological processes, including tumor angiogenesis. The expression and activation of Stat3 in the mouse model of ischemia-induced retinal neovascularization was investigated to evaluate the possible role of STAT3 in retinal vascular disease. METHODS Retinal neovascula...

We report for the first time low-level quinolone resistance mediated by decreased expression of topoisomerase IV in Staphylococcus aureus. A single-step mutant of wild-type S. aureus strain ISP794, P18 selected by using twice the MIC of premafloxacin, had four- and four- to eightfold greater MICs of premafloxacin and ciprofloxacin, respectively, than the wild type. Sequencing of parEC and gyrBA...

p18 is a member of the INK4 family of proteins that regulate the G1 to S cell cycle transition by binding to and inhibiting the pRb kinase activity of cyclindependent kinases 4 and 6. The p16 member of the INK4 protein family is altered in a variety of cancers and structure-function studies of the INK4 proteins reveal that the vast majority of missense tumor-derived p16 mutations reduce protein...

The accurate study of aerosol composition and nicotine release by electronic cigarettes is a major issue. In order to fully and correctly characterize aerosol, emission generation has to be completely mastered. This study describes an original vaping machine named Universal System for Analysis of Vaping (U-SAV), dedicated to vaping product study, enabling the control and real-time monitoring of...

Antibiotic is one of the greatest discoveries in human history. It has drastically promoted modern medicine and extended average lifespan. However, antibiotic resistance become a global crisis today development novel antibiotics highly demanded. The traditional not only kill pathogen but also damage resident microbiome body, thus promoting elevating risk patients for new infection. Here, we fab...

Glioblastoma multiforme (GBM) is one of the most dreaded cancer diagnoses due to its poor prognosis and the limited treatment options. Homozygous deletion of the p16/ p14/p15 locus is among the most common genetic alterations in GBM. Two recent studies have shown that deletion and mutation of another INK4 family member, p18, also drives the pathogenesis of GBM. This minireview will discuss the ...

INTRODUCTION Culture methods used to determine Escherichia coli levels in food products are time consuming and labor intensive. The TEMPO system and reagents have been developed to replace plate and tube counting methods. In this study the TEMPO system has been used to compare the performance of the TEMPO EC test for the enumeration of E. coli in food products to the ISO 16649-2 plate count met...

The two authors of this paper belong to the expert commission of the standardization bodies in France (AFNOR) and in Germany (DIN) and are, within the ISO/TC37/SC2, project leader and expert for the revision of the ISO-standard. In this paper we will report on the revision of the standard ISO 1951 Presentation/Representation of entries in dictionaries which will give recommendation regarding th...

In human platelets, thrombin not only stimulates the phosphorylation of pleckstrin (P47) and of myosin P-light chains, but also induces the dephosphorylation of an 18-19 kDa phosphoprotein (P18) [Imaoka, Lynham and Haslam (1983) J. Biol. Chem. 258, 11404-11414]. We have now studied this protein in detail. The thrombin-induced dephosphorylation reaction did not begin until the phosphorylation of...

The Eustachian tube is a small canal that connects the tympanic cavity with the nasal part of the pharynx. The epithelial lining of the Eustachian tube contains a ciliated columnar epithelium at the tympanic cavity and a pseudostratified, ciliated columnar epithelium with goblet cells near the pharynx. The tube serves to equalize air pressure across the eardrum and drains mucus away from the mi...