To simplify the construction of monoclonal antibody (MAb) libraries to complex biological systems, the use of a cascade procedure has been investigated. The procedure continually restricts MAb production to those antigens previously so unidentified. Murine macrophage membranes were detergent-solubilized and purified by Lens culinaris lectin affinity chromatography. Prior to immunization of rats...

Damping off caused by Rhizoctonia solani (isolate AG4) constrains growing soybean in the north Iranian province of Golestan. The pathogen was grown on malt agar containing 0, 50, 100 or 200 ppm trifluralin then its vegetative growth and virulence were evaluated. The response of soybean cv. Williams seedlings grown in soil containing 0, 0.5, 1 and 2 ?g ai g-1 trifluralin was investigated. On mal...

By using mixed rigid and flexible organic ligands, three Keggin based compounds, [Ag6(tbz)4(bmz)2(H3PW12O40)]·4H2O (1), [Ag(H2bdpm)2(H2PMo12O40)]·7.5H2O (2), and [Ag4(tbz)2(H2bdpm)2(HPMo12O40)]·2H2O (3) (Htbz = thiabendazole, Hbmz = benzimidazole, H2bdpm = 1,1'-bis(3,5-dimethyl-1H-pyrazolate)methane), were hydrothermally synthesized and structurally characterized. Through adopting rigid Htbz li...

A convenient method for the synthesis of silver alkynyl compounds containing different nuclearities has been developed. The reaction of AgC[triple bond, length as m-dash]CBu(t) and AgX (X = CF3CO2(-), C2F5CO2(-)) in the presence of auxiliary ligands L (L = bipyridine, bipy; 1,10-phenanthroline, phen; 2-(diphenylphosphino)pyridine, dppy) under different conditions afforded a family of new silver...

ated colon cancer, the 5 mm after iv. injection 10.4 nmol/breast fluid sample with a mea@i of 2.04 ±2.86 for 11 randomly selected specimens. As th4'e are 100 nmol of histidine normally present in the top ag4' used in the Ames test, our values averaged only 2% and n@tmore than I 0% of increased histidine. We found that to incr@asethe number of spontaneous nevertant mutations above @heaverage va...

Chemicals typically available in plants have the capability to reduce silver and gold salts and to create silver and gold nanoparticles. We report the preparation of silver nanoparticles with sizes between 10 and 300 nm from silver nitrate using fruit extract collected from pineapples and oranges as reducing agents. The evolvement of a characteristic surface plasmon extinction spectrum in the r...

A monoclonal antibody (BA11) has been produced against Clostridium botulinum type A neurotoxin by the fusion of myeloma cells (P3 NS1/1-Ag4-1) with spleen cells from BALB/c mice immunized with botulinum type A neurotoxoid. The antibody bound specifically to botulinum type A neurotoxin, showing no cross-reactivity with types B and E botulinum toxins or with any of several other bacterial toxins ...