background: methicillin resistant staphylococcus aureus (mrsa) has been emerged as a nosocomial and community acquired pathogen worldwide. there are many challenges for laboratory detection of mrsa. the aim of this study was to compare different phenotypic methods with pcr based method as a gold standard for detection of meca gene. methods: a total of 220 clinical isolates of s. aureus which we...

A total of 245 isolates of Escherichia coli (paracolons) were employed in comparing the use of acetate differential agar (ADA) with the simple test described by Sellers to eliminate these organisms from consideration as enteric pathogens. At 18 hr after the media were inoculated, 83% of the isolates produced a positive citrate reaction on Sellers citrate-mannitol-agar (SCM) and 7% gave a positi...

Difference in expression of putative virulence factors and in antifungal susceptibility among different Candida species has raised the need for species-level identification. The close relationship of Candida dubliniensis with C. albicans has led to misidentification of C. dubliniensis isolates as C. albicans. Phenotypic tests include ability to produce chlamydospore on casein agar, colony colou...

1. As found by Sordelli and Mayer, agar adsorbed on bacteria produces agar-reacting antibodies in animals injected with these organisms. 2. False cross-agglutination and precipitin reactions can be produced in sera containing agar-reacting antibodies by organisms grown on agar. Zozaya's publication in this field (3) requires partial revision on this account. 3. There is suggestive immunological...