A.GAROSE, tile niain compollent of agar, has recently become of great interest in biologic research. This interest is due to the superior meellanical qualities of agarose-gels at low concentrations aI1d to their neutral character. Agarose has found applicatioll as a useful matrix for immunodiffusion (1), electrophoresis (2-4) and as an overlay for VjruS titrations with the ‘‘plaque tecilIliqile...

OBJECTIVE(S) Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. MATERIALS AND METHODS In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose...

We explored the possibility of using ionic liquids (ILs) as medium for efficient extraction of agarose via dissolution of red algae under varying conditions of heating or microwave irradiation. As compared to conventional methods, a very high extraction yield of good quality agarose (as high as 39 wt%) could be achieved depending upon the nature of used IL and applied experimental conditions. P...

1. Enzymically active insoluble derivatives of chymotrypsin and rennin were prepared by coupling each enzyme to agarose as described by Porath, Axén & Ernback (1967) and rennin to aminoethylcellulose by the method of Habeeb (1967). 2. Agarose-chymotrypsin was stable over the range pH2-9, but agarose-rennin released active enzyme into solution at above pH2 and aminoethylcellulose-rennin was simi...

Metal-enhanced fluorescence of conjugated polyelectrolytes (CPs) is realized using a simple, green hybrid Ag nanocomposite film. Ag nanoparticles (Ag NPs) are pre-prepared by sodium citrate reduction and incorporated into agarose by mixing to form an Ag-containing agarose film (Ag@agarose). Through variation of the amount of Ag NPs in the Ag@agarose film as well as the thickness of the interlay...

The optical properties of agarose thin films, such as absorption, absorption coefficient, and extinction are presented in this study. To obtain a 2% wt/v concentration agarose, 0.2 mg was dissolved 10 mL water, the mixture then heated microwave for 30 second until boiling. In current investigation, three types water were used, including distilled labeled W1 (pH = 7.5 TDS 18 ppm), Zamzam W2 8 33...

Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. On the basis of previous estimates of the sugar content of the hormone, six lectins (wheat germ agglutinin, phytohemagglutinin, Ricinus communis 120, soybean agglutinin, concanavalin A, and limulin) were chosen for study. Only wheat ger...