BACKGROUND Vitamin D deficiency remains common in children and adults in Pakistan despite adequate sunlight exposure. Diagnosis in adults is usually delayed and is made following pathological fractures that result in significant morbidity. The objective of this study was to see whether Serum Alkaline Phosphatase levels could be used as a screening test for osteomalacia. METHODS The Study was ...

Macromolecular alkaline phosphatase (EC 3.1.3.1) was found in the serum of a patient suffering from myasthenia gravis (adult type II) complicated with thymoma, and was shown by immunoelectrophoresis to be bound to immunoglobulins A and G (IgG). Placental alkaline phosphatase, complexed with either the patient's serum or IgG purified from the patient's serum, remained at the origin on electropho...

BACKGROUND AND OBJECTIVES Recent in vitro studies have shown a link between alkaline phosphatase and vascular calcification in patients with chronic kidney disease (CKD). High serum levels of alkaline phosphatase are associated with increased death risk in epidemiologic studies of maintenance hemodialysis (MHD) patients. We hypothesized that coronary artery calcification is independently associ...

Background: The most frequent sites of metastases in prostatic carcinoma are bones. Bone scintigraphy is the diagnostic tool of first choice for staging bone metastases in cancer of the prostate. But this examination is expensive and time-consuming.Therefore we are looking especially for serum parameters like alkaline phosphatase, the isoenzyme of bone-specific alkaline phosphatase, and prostat...

soil enzyme activities can be used as indicators of soil quality for assessing the sustainability of agricultural ecosystems. the objective of this study was to determine the effects of barley residue input rate, burning, urea fertilizer and tillage management on activities of alkaline phosphatase and urease under field conditions, after a period of 90 days. the experiment was carried out based...

Bile duct ligation causes a five- to sevenfold increase in the activity of rat liver alkaline phosphatase within 12 hours after ligation and a similar rise in the activity of alkaline phosphatase in serum. The increased serum activity is due entirely to the appearance of a new isoenzyme that has the properties of rat liver alkaline phosphatase. The increase in both serum and liver alkaline phos...

Serum total alkaline phosphatase is the most commonly used biochemical marker of bone disease in renal patients, but alkaline phosphatase originates from different organs and sometimes lacks specificity. Bone isoenzyme measurement is considered superior to total alkaline phosphatase for the assessment of bone metabolism. We have studied the value of bone isoenzyme, determined by a new. IRMA (Ta...

Background: Collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. This feature of the collagen renders  establishment of the structural skeleton in tissues. Regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...

alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. in the present study, alkaline phosphatase gene was inserted into the pet15b vector. the recombinant dna was then expressed using iptg as an inducer. the expression of this enzyme was optimized by changing expression conditions...

This study assessed the utility of serum alkaline phosphatase as a screening test to identify vitamin D deficiency and documented that it was not a useful screening tool.