Herpesviruses encode a unique serine protease essential for viral capsid maturation. This protease undergoes autoprocessing at two sites, R and M, at the consensus sequence (V, L, I)(P3)-X(P2)-A(P1)/S(P1') (where X is a polar amino acid). We observed complete autoprocessing at the R and M sites of Marek's disease virus (MDV) protease following production of the polyprotein in Escherichia coli. ...

Structural origin of substrate-enzyme recognition remains incompletely understood. In the model enzyme system of serine protease, canonical anti-parallel beta-structure substrate-enzyme complex is the predominant hypothesis for the substrate-enzyme interaction at the atomic level. We used factor Xa (fXa), a key serine protease of the coagulation system, as a model enzyme to test the canonical c...

Herein, we report on the synthesis of 4 novel pyridine derivatives (P1, 2, 3 and 4) with differential serine protease inhibitory effects utilizing pyridine-2 (1H)-ones as starting materials. When these 4 compounds were tried to inhibit cercarial serine protease, the major penetration enzyme of the parasite Schistosoma mansoni, P1 and P3 exerted the most potent inhibitory effects as demonstrated...

background: hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albendazole (abz) and mebendazole (mbz). the purpose of this study was comparing the effects of abz and mbz on glutathione-s-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst. methods: the culture supernatants containing the parasite excretory...

In vitro experiments were conducted to examine the characteristics and mode of action of a protease that increased the ruminal fiber digestibility of alfalfa hay. A commercial source of protease (Protex 6L, Genencor Int., Rochester, NY), already characterized for its main activities, was further analyzed to determine protease activity in response to pH, molecular size by SDS-PAGE, specificity t...

Abstract The maintenance of hemostasis to ensure vascular integrity is dependent upon the rapid conversion zymogen species coagulation cascade their enzymatically active forms. This process culminates in generation serine protease thrombin and polymerization fibrin prevent leak at sites endothelial cell injury or loss cellular junctions. Thrombin can be initiated by extrinsic pathway through ex...