Washed membranes of bovine adrenal chromaffin granules contained most of the cholesterol and phospholipids of the particle and 22% of the total protein. The protein/lipid ratio was about 0.45 (w/w). Dopamine(3,4-dihydroxyphenethylamine)beta-hydroxylase, Mg(2+)-activated nucleoside triphosphatase and cytochrome b-559 activities were present in the membrane. ATP was the best substrate for the nuc...

In this study, we investigated ways by which to optimize metals and nutrients solubilization from sewage sludge using alkaline–thermal hydrolysis the Box–Behnken design. We also examined through struvite crystallization recovery of solubilized hydrolyzed liquid determined effect NaOH concentration, liquid/solid ratio, temperature on process. Nutrients was positively affected decreasing liquid/s...

A study was conducted to isolate and characterize phosphate solubilizing bacteria (PSB) from soils of various locations in North Gujarat. total 40 PSB isolates were obtained, exhibiting a index (PSI) ranging 2.11 4.33. Interestingly, among isolates, subset 16 displayed an additional trait potassium solubilization, as evidenced by their (KSI) values falling within the range 2.10 3.10. These scre...

Isoelectric solubilization /precipitation (ISP) process was used to isolate protein from muscles of Prussian carp, Carassius gibelio (Bloch, 1782). Fish protein and lipid were recovered from whole gutted Prussian carp using acidic and basic isoelectric solubilization/precipitation followed by assaying amino acid and fatty acid profile. Essential amino acids content in acidic and basic pH treatm...

The core objective of this analysis is to implement a combination alkaline (NaOH) and sonication pretreatment techniques produce energy-efficient biohydrogen from the marine macroalgae Chaetomorpha antennina. Anaerobic fermentation was implemented in control, sonic solubilization (SS) alkali (SAS) for 15 days. In production 40 mL H2/gCOD obtained. sonicator intensities varied 10% 90% period 1 h...

Response surface methodology (RSM) was adopted for the optimization of process variables in the alkaline peroxide oxidation (APO) pretreatment of Vitellaria paradoxa sawdust based on central composite design (CCD) experiments. A 2 five level CCD with central and axial points was used to develop a statistical model for the optimization of process variables. Maximum response for the pretreatment ...

Solubilization of sewage sludge creates favorable conditions for anaerobic microorganisms to produce biogas. In this paper, we quantify the effect of heating pretreatment on the degree of solubilization of sewage sludge. The pretreatment process was carried out using a lab-scale industrial microwave unit (2450 MHz frequency). Response surface analysis was applied to determine the combination of...

Eight strains isolated from the stems of Lippia sidoides were identified as belonging to Lactococcus lactis, a bacterial species considered as "generally recognized as safe". Their capacity to solubilize/mineralize phosphate was tested in vitro with different inorganic and organic phosphorus (P) sources. All strains were able to solubilize calcium phosphate as an inorganic P source, and the bes...

Vegetative Bacillus thuringiensis subsp. israelensis cells (6 X 10(5)/ml) achieved 100% mortality of Aedes aegypti larvae within 24 h. This larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. However, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during ...