1. It is likely that certain tailed and filamentous particles seen on electron microscope examination of partially purified saline suspensions of Newcastle virus are the individual virus particles because: (a) They have a highly characteristic shape not seen in other virus preparations. (b) They are present whenever the virus is present in high concentration. (c) Their size agrees with the size...

Histochemical methods demonstrate the accumulation of two types of mucous inclusion in the allantoic epithelial cells of the chick embryo. One type of inclusion contains a sialomucin that is PAS-reactive, unstained by Alcian blue and other anionophilic dyes and is susceptible to digestion by Vibrio choleme neuraminidase. The second inclusion contains an apparent sulfomucin which is Alcianophili...

A trypsin-induced hemagglutination (THA) assay was standardized to detect infectious bronchitis virus (IBV) in allantoic fluid (AF) of embryonated eggs. The test was used in 20 samples, each collected from 5 different layer farms suspected for IBV. Allantoic fluid from inoculated embryos was harvested and treated with reagent grade trypsin at the percentages of 0.25, 0.50, 1.0 and 2.0 for 30 mi...

During a study on high mortality cases of goose embryo in Shandong Province, China (2014-2015), we isolated an H9N2 avian influenza virus (AIV) strain (A/goose/Shandong/DP01/2014, DP01), which was supposedly the causative agent for goose embryo death. Sequence analysis revealed that DP01 shared 99.9% homology in the HA gene with a classic immune suppression strain SD06. To study the potential v...

Highly purified preparations of PR8 influenza virus were obtained from perfused, infected mouse lungs by a combination of methods involving adsorption of the virus on and elution from chicken red cells and differential centrifugation. Such preparations were found to possess 50 per cent infectivity end-points at 10(-11) to 10(-11.8), and 10(-13) to 10(-14.3) gm. of nitrogen in mice and in chick ...

Development within the cleidoic egg of birds and reptiles presents the embryo with the problem of accumulation of wastes from nitrogen metabolism. Ammonia derived from protein catabolism is converted into the less toxic product urea or relatively insoluble uric acid. The pattern of nitrogen excretion of the green iguana, Iguana iguana, was determined during embryonic development using samples f...

Quail allantoic endoderm was implanted into the presumptive digestive-tract area of chick embryos, and the differentiation of the endoderm was examined morphologically and immunocytochemically with antisera against pepsinogens and sucrase. The allantoic endoderm was incorporated into the host digestive organs. It often became continuous with the host endoderm and formed a chimaeric digestive-tr...

The results of determining the parameters chicken embryos for obtaining inactivated influenza vaccines are presented. studies were carried out at LLC "Fort" (Ryazan region). 8.736 million used from chickens egg crosses Rhodonit-3, Hisex brown. In course research, dependence development a embryo on various growth periods was revealed. It has been established that optimal use in production life s...

Hydrops conditions of the placenta in the mare are uncommon, with hydrops allantois being reported more frequently than hydrops amnion. The condition in the mare usually develops during the last trimester of pregnancy and is characterized by the excessive accumulation of allantoic (hydrops allantois) or amnionic fluid (hydrops amnion). The sequelae of unnoticed or untreated hydrops allantois/ a...

Cortisone has been found by Schwartzman (Proc. Soc. Exptl. Biol. Med., 75, 835, 1950) and by Kilbourne and Horsfail (Proc. Soc. Exptl. Biol. Med., 76, 116, 1951) to increase the susceptibility of the host to certain viruses. Studies were undertaken therefore to determine whether the administration of cortisone might render chick embryos susceptible to infection with the Lansing strain of poliom...